CAP-Stimulated Media Composition for Stable Anti-Cancer Storage
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Solution Overview
Problem
Cold atmospheric plasma-stimulated media (PSM) degrades during storage at recommended temperatures between 2° C. and 8° C., compromising its anti-cancer activity, necessitating a solution for enhanced stability and retention of therapeutic potential.
Innovation Solution
Incorporation of a tyrosine derivative, such as 3-nitro-L-tyrosine, into the medium, along with adjustments to reduce components like cysteine, methionine, phenylalanine, and phenol red, stabilizes reactive species like H2O2, maintaining anti-cancer activity at temperatures between −25° C. and 25° C. for up to 7 days.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Temperature
If PSM is stored at recommended temperatures between 2°C and 8°C, then the medium maintains ideal storage conditions, but the medium degrades and loses anti-cancer capacity over time
Solution Approach 1:
The patent modifies the chemical composition parameters of the medium by removing specific components (cysteine, methionine, phenylalanine, phenol red) and adding stabilizing agents (tyrosine derivatives like 3-nitro-L-tyrosine, ascorbic acid). These parameter changes enable the medium to maintain stability at refrigerated temperatures without freezing, resolving the contradiction between ideal storage temperature and capacity retention.
Solution Approach 2:
The patent introduces intermediary substances (tyrosine derivatives and ascorbic acid) that act as stabilizers between the reactive species in the plasma-stimulated medium and the degradation pathways. These intermediaries prevent decomposition reactions, allowing the medium to maintain its anti-cancer capacity during storage at 2°C to 8°C without requiring freezing conditions.
2Stability of the object's composition
If PSM is frozen at -80°C to inhibit degradation, then stability is improved, but the medium requires freezing conditions that are not ideal for clinical storage
Solution Approach 1:
The patent changes the chemical parameters of the medium by formulating it without cysteine, methionine, phenylalanine, and phenol red, and by adding tyrosine derivatives and ascorbic acid. These compositional changes fundamentally alter the stability parameters, enabling the medium to remain stable at refrigerated temperatures (2°C to 8°C) without requiring freezing at -80°C, thus resolving the contradiction between stability and storage temperature requirements.
3Ease of operation
If CAPSM is stored at temperatures between 2°C and 8°C for extended periods, then storage convenience is improved, but anti-cancer activity degrades over time
Solution Approach 1:
The patent introduces tyrosine derivatives (such as 3-nitro-L-tyrosine) and ascorbic acid as intermediary stabilizing agents that protect the reactive species in the CAPSM from degradation. These intermediaries extend the duration of anti-cancer activity while allowing storage at convenient refrigerated temperatures (2°C to 8°C), resolving the contradiction between storage convenience and activity duration.
Solution Approach 2:
The patent performs preliminary stabilization by formulating the medium with protective agents (tyrosine derivatives and ascorbic acid) before storage. This preliminary action prevents degradation during storage, allowing the medium to maintain its anti-cancer activity for extended periods (up to 7 days or more) at convenient refrigerated temperatures, thus resolving the contradiction between ease of operation and duration of action.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The stabilized medium retains anti-cancer capacity and stability, effectively inhibiting cancer cell growth over extended storage periods without freezing, enhancing clinical applicability.
Implementation Method 1
cold atmospheric plasma (CAP) has shown a selective anti-cancer capacity
Implementation Method 2
both reactive oxygen species (ROS) such as hydroxyl free radicals (OH) and hydrogen peroxide (H2O2) and reactive nitrogen species (RNS) such as nitric oxide (NO) and nitrite (NO2) are dissolved in the aqueous solution
Implementation Method 3
H2O2 has been found to mainly contribute to the death of cancer cells after the direct CAP irradiation on cancer cells or the indirect CAP irradiation on the culture medium
Data Source
AI summary
This disclosure relates to stabilized anti-cancer atmospheric plasma (CAP)-stimulated media, to methods for preparing such media, and to methods of treatment using such media.