Capillary Array Electrophoresis Chemiluminescence Detection System
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Solution Overview
Problem
Current capillary array electrophoresis (CAE) detection systems face limitations due to fluorescence and scattering light signal interference, mechanical scanning inefficiencies, and low sensitivity, particularly in detecting low-concentration components, which hampers bioanalysis, clinical diagnosis, and environmental monitoring.
Innovation Solution
A CAE-chemiluminescence (CL) detection coupled system is introduced, eliminating the need for a light source by using a capillary array with a chemiluminescent reaction tank, imaging lens, and array detectors to capture chemiluminescent signals, enhancing sensitivity and throughput without mechanical scanning.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Area of stationary object
If a laser fluorescence detector is used in imaging mode to detect capillary array, then detection coverage is improved, but fluorescence signal interference between adjacent capillaries occurs
Solution Approach 1:
The patent extracts and removes the light source from the detection system, eliminating the excitation source that causes fluorescence and scattering light signal interference. By using chemiluminescence reaction instead of laser excitation, the system achieves detection without the harmful light interference while maintaining comprehensive capillary array coverage.
2Object-affected harmful factors
If a laser fluorescence detector operates in scanning mode to eliminate signal interference, then signal interference is reduced, but data collection efficiency deteriorates due to mechanical movement
Solution Approach 1:
The patent replaces the mechanical scanning system with a stationary chemiluminescence detection system. Instead of mechanically moving the laser beam or capillary array to scan individual capillaries, the system uses chemical reactions that naturally produce light at each capillary outlet, allowing simultaneous detection of all capillaries without mechanical movement, thus eliminating both signal interference and mechanical scanning inefficiencies.
3Object-affected harmful factors
If UV-vis detector uses fiber optic detection mode to avoid scattering light interference, then signal interference is reduced, but sensitivity and throughput deteriorate
Solution Approach 1:
The patent changes the detection parameter from optical absorption (UV-vis) to light emission (chemiluminescence). By using chemiluminescent reagents that produce intense light through chemical reactions, the system achieves both elimination of scattering light interference and enhanced sensitivity, as chemiluminescence signals are inherently brighter and more detectable than absorbed UV-vis signals.
4Adaptability or versatility
If existing CAE detectors use light source as excitation source to detect sample signal, then detection capability is achieved, but background signal increases and sensitivity reduces
Solution Approach 1:
The patent extracts and removes the light source from the detection system, eliminating the excitation source that generates background signals such as Rayleigh scattering, Raman scattering, and solvent fluorescence. By using chemiluminescence reaction where the sample itself produces light through chemical reaction with chemiluminescent reagents, the system achieves detection capability without background signal interference, dramatically improving sensitivity for low-concentration component detection.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The CAE-CL system achieves high sensitivity and rapid detection of low-concentration components with improved throughput, simplifying the structure and reducing costs, making it suitable for bioanalysis, clinical diagnostics, and environmental monitoring.
Implementation Method 1
Capillary electrophoresis (CE) is an efficient separation and analysis method based on the difference in charge-mass ratio of individual components using capillary as the electrolyte support and a high-voltage electric field as the driving force
Implementation Method 2
A sample flows out from the outlet end of the capillary array, and then reacts with a chemical reagent to generate CL
Implementation Method 3
The CL is collected by the imaging lens
Implementation Method 4
the plane array or linear array detector converts an optical signal into an electrical signal
Data Source
AI summary
A capillary array electrophoresis (CAE)-chemiluminescence (CL) detection coupled system includes a high-voltage power supply, a capillary array, an array channel CL reaction tank, a CAE sample tank, a CAE detection tank, a chemiluminescent reagent delivery unit, a multi-channel detection unit, and a data acquisition and processing unit. An inlet end of the capillary array is connected to the CAE sample tank. An outlet end of the capillary array is connected to the array channel CL reaction tank, and is further connected to the CAE detection tank.