Capillary Electrophoresis Aggregate Removal for Precision Analysis
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Solution Overview
Problem
Current analysis methods, such as electrophoresis, face challenges in achieving precise analysis due to the presence of aggregates formed between sample components and additional components in the electrophoretic liquid, which can lead to noise in measurement results and reduced precision.
Innovation Solution
A capillary electrophoresis method that involves mixing the sample with an anionic group-containing compound, removing aggregates through filtering or centrifugal separation, and performing electrophoresis in a capillary tube where the analysis component and the anionic group-containing compound are bound, ensuring continuous sample supply and improved precision.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If additional components are added to the electrophoretic liquid to improve analysis precision, then measurement accuracy is improved, but aggregates form between sample components and additional components causing noise in measurement results
Solution Approach 1:
The patent applies preliminary action by performing aggregation removal before the electrophoresis measurement process. The additional components are added to the electrophoretic liquid in advance, allowing aggregates to form during sample mixing, and then these aggregates are removed through filtration or centrifugal separation before the actual analysis begins. This prevents noise during measurement while maintaining the precision-enhancing effects of the additional components during the measurement phase.
Solution Approach 2:
The patent segments the analysis process into distinct stages: (1) adding additional components to the electrophoretic liquid, (2) mixing sample with electrophoretic liquid to form aggregates, (3) removing aggregates through filtration or centrifugal separation, and (4) performing electrophoresis measurement. This segmentation allows the system to benefit from additional components during measurement while eliminating their harmful aggregate effects in between stages.
2Device complexity
If traditional electrophoresis methods are used without aggregate removal, then the analysis process is simpler, but measurement precision is reduced due to noise from aggregates
Solution Approach 1:
The patent introduces intermediary devices and processes between sample introduction and measurement: filtration means (such as filters or membrane filters) or centrifugal separation means. These intermediaries remove aggregates from the sample-electrophoretic liquid mixture before the mixture enters the capillary tube for electrophoresis measurement, thereby eliminating noise without significantly complicating the overall device structure.
Solution Approach 2:
The patent extracts the harmful aggregate components from the sample-electrophoretic liquid mixture through filtration or centrifugal separation. By taking out the aggregates (the harmful portion) while retaining the useful sample components and electrophoretic liquid, the system maintains measurement precision without requiring complete redesign of the analysis process.
3Volume of moving object
If continuous sample supply is implemented during electrophoresis, then smaller chip sizes can be used, but aggregate formation may increase with continuous mixing
Solution Approach 1:
The patent performs aggregate removal as a preliminary action before the continuous supply and electrophoresis measurement. By filtering or centrifuging the sample-electrophoretic liquid mixture beforehand, the system eliminates aggregates that would otherwise form during continuous mixing, enabling small chip size without the penalty of increased noise from aggregate formation.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach suppresses noise in measurement results, enhancing analysis precision and allowing for the use of smaller analysis chip sizes, making the method more efficient and reliable.
Implementation Method 1
a complex in which the analysis component and the anionic group-containing compound are bound to each other
Implementation Method 2
performing electrophoresis in a capillary tube where the electrophoresis is performed with a complex
Implementation Method 3
in the removing step, the aggregate is removed by filtering
Implementation Method 4
in the removing step, the aggregate is removed by centrifugal separation
Data Source
AI summary
A method for analyzing a sample using capillary electrophoresis is provided. By the method, following steps are performed. First, an original sample and an anionic group-containing compound are fixed to form a mixed sample, where the original sample contains an analysis component to be analyzed and a sub component other than the analysis component. Then, an aggregate of the sub component and the anionic group-containing compound is removed from the mixed sample. Then, electrophoresis is performed in a capillary tube with respect to a complex in which the analysis component and the anionic group-containing compound are bound to each other, while the mixed sample is continuously supplied.