Capillary Electrophoresis Interface Control by Preliminary Pressurization
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Solution Overview
Problem
The formation of an interface between the sample and electrophoresis liquid is often incomplete in capillary electrophoresis, leading to deteriorated analysis accuracy due to mixing and separation issues.
Innovation Solution
A method involving pressurization of the electrophoresis liquid in the capillary flow path followed by a waiting period to form a stable interface before applying a voltage for separation, ensuring accurate analysis.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of operation
If the capillary tube is filled with electrophoresis liquid and sample is introduced into the feed tank, then the sample can be made to flow inside the feed tank to generate shear flow, but the interface between sample and electrophoresis liquid may not be formed well and analysis accuracy deteriorates
Solution Approach 1:
The electrophoresis liquid is pressurized into the capillary flow path before sample introduction to pre-establish the liquid interface. This preliminary action ensures that when the sample is subsequently introduced into the feed tank, a clear and stable interface is already in place, preventing mixing and maintaining high analysis accuracy.
Solution Approach 2:
Pressure control is used to regulate the electrophoresis liquid flow into the capillary flow path. By applying pressure to the electrophoresis liquid reservoir, the system precisely controls the liquid interface formation between the electrophoresis liquid and sample, ensuring a sharp boundary that improves analysis accuracy.
2Productivity
If sample is introduced into feed tank connected to capillary tube, then sample can be analyzed by electrophoresis, but part of sample may move into capillary tube and interface formation is compromised
Solution Approach 1:
The system performs preliminary pressurization of electrophoresis liquid into the capillary flow path before sample introduction. This pre-establishes a controlled liquid interface that prevents sample from improperly entering the capillary tube, ensuring high interface formation quality while maintaining efficient analysis throughput.
Solution Approach 2:
The system changes the pressure parameter of the electrophoresis liquid to control interface formation. By adjusting pressure, the system optimizes both the sharpness of the liquid interface and the efficiency of sample analysis, resolving the contradiction between throughput and precision.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enhances analysis accuracy by forming a clear interface between the electrophoresis liquid and sample, allowing precise separation and detection of components.
Implementation Method 1
a pressurization process of pressurizing the first liquid into the capillary flow path
Implementation Method 2
a separation process of applying a voltage between the sample reservoir storing the second liquid and the capillary flow path filled with the first liquid, so that components in the sample contained in the second liquid move in the capillary flow path and the components are separated in the capillary flow path
Data Source
Figure 1(a)~1(b)
Figure 2(a)~2(c)
Figure 3
AI summary
An analysis method using a microchip 100 which is provided with a capillary flow path 2, and a sample reservoir 1 connected to the capillary flow path 2, in which the capillary flow path 2 is filled with a first liquid for electrophoresis, and a second liquid containing a sample is stored in the sample reservoir, and including a pressurization process in which the first liquid is pressurized into the capillary flow path 2 from a side of the capillary flow path that is opposite from the side connected to the sample reservoir, and a separation process in which a voltage is applied between the sample reservoir 1 storing the second liquid and the capillary flow path 2 filled with the first liquid, such that components in the sample contained in the second liquid move in the capillary flow path and the components are separated in the capillary flow path.