Capillary Electrophoresis Apparatus Position Correction
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Solution Overview
Problem
In capillary electrophoresis apparatuses, the use of capillaries as both excitation and detection units leads to position shifts during capillary exchange, causing wavelength shifts and pull-up signals that reduce analysis reliability, and existing methods require spectral data acquisition with each capillary exchange, compromising sensitivity and data acquisition simultaneity.
Innovation Solution
A capillary electrophoresis apparatus that includes a capillary position measuring light source to detect capillary position deviations, allowing for the correction of spectral data and setting of data acquisition areas, eliminating the need for spectral data acquisition with each capillary exchange and maintaining sensitivity and data acquisition simultaneity.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If spectral data acquisition is performed with each capillary exchange, then wavelength shift accuracy is improved, but analysis time increases and sensitivity is compromised
Solution Approach 1:
The patent applies preliminary action by pre-acquiring spectral data during capillary manufacturing or initial setup, storing it for later reuse. This eliminates the need to acquire spectral data during each capillary exchange, thereby reducing analysis time while maintaining wavelength accuracy through the use of pre-stored reference data
Solution Approach 2:
The patent uses copying by creating a digital copy of the spectral data from a reference capillary and applying it to subsequent capillaries. Instead of re-acquiring spectral data for each capillary exchange, the system copies the wavelength characteristics from the reference measurement, significantly reducing analysis time while maintaining measurement precision
2Device complexity
If capillary position is not monitored, then device complexity is reduced, but analysis reliability deteriorates due to pull-up signals
Solution Approach 1:
The patent implements feedback by using a position detection unit to monitor capillary position in real-time and feeding this information back to the control unit. The control unit then adjusts the spectral data or correction parameters based on the detected position, eliminating pull-up signals and improving analysis reliability without significantly increasing device complexity
Solution Approach 2:
The patent replaces complex mechanical positioning systems with an optical or electromagnetic detection system. Instead of using precision mechanical stages to maintain capillary position, the system uses a position detection unit (optical sensor or camera) to detect position and uses computational methods to correct for position variations, simplifying the mechanical system while improving reliability
3Ease of operation
If capillary position shifts are not corrected, then operational complexity is reduced, but spectral data accuracy deteriorates
Solution Approach 1:
The patent applies self-service by enabling the system to automatically detect capillary position shifts and correct spectral data without operator intervention. The position detection unit and control unit work together to automatically adjust wavelength calibration and correct spectral data, maintaining measurement precision while keeping operational simplicity
Solution Approach 2:
The patent uses parameter changes by dynamically adjusting spectral parameters (wavelength calibration, correction factors) based on detected capillary position. When position shifts are detected, the control unit modifies the spectral data parameters to compensate for the shift, maintaining accuracy without requiring complex manual recalibration procedures
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The apparatus effectively reduces pull-up signals and maintains analysis reliability by measuring and correcting capillary position shifts, ensuring consistent spectral data acquisition without the need for repeated spectral data acquisition during capillary exchanges.
Implementation Method 1
a capillary image is detected with a two-dimensional detector to determine a capillary position deviation
Implementation Method 2
a capillary position measuring light source. A capillary is irradiated with light from capillary position measuring light source
Data Source
AI summary
An object of the present invention is to provide a capillary electrophoresis apparatus in which simultaneity can be ensured between sensitivity and data acquisition to decrease a pull-up signal while spectral data acquisition is eliminated in each capillary exchange. The invention relates to a capillary electrophoresis apparatus in which capillary position shift is detected in each capillary exchange by detecting a capillary position. A capillary position measuring light source is provided in the capillary electrophoresis apparatus of the invention. The capillary is irradiated with light emitted from the capillary position measuring light source, a capillary image is detected with a two-dimensional detector, and thereby a position deviation of the capillary is determined. On the basis of the position deviation of the capillary, a data acquisition area is set in the two-dimensional detector, or a reference fluorescent light spectrum determined from the capillary at the standard position is corrected.