Capillary Microplate for Discrete Sample Partitioning
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Solution Overview
Problem
Current methods for microbial concentration and quantification, such as pour plate and membrane filtration, face challenges like small or overlapping bacterial colonies, particulate interference, and require skilled personnel, limiting accuracy and ease of use.
Innovation Solution
A device utilizing capillary flow to partition a liquefied sample into discrete compartments, reducing hands-on time and eliminating the need for skilled personnel, with a microbiological medium that facilitates growth and visible detection of target microorganisms.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If pour plate method is used, then microbial quantification can be performed, but bacterial colonies may be too small or overlapping making counting difficult
Solution Approach 1:
The device divides the sample distribution into multiple discrete compartments (50-100 wells), each containing a portion of the sample. This segmentation prevents colony overlapping by isolating bacteria in separate compartments, making counting easier while maintaining statistical accuracy for microbial quantification.
2Measurement precision
If membrane filtration method is used, then microbial detection can be performed, but particulate matter may clog the membrane making it unusable
Solution Approach 1:
The device extracts and removes the problematic filtration step entirely. Instead of forcing samples through membranes that clog with particulates, the invention uses passive capillary action to distribute liquid samples directly into compartments, eliminating membrane clogging while maintaining microbial detection capability.
3Measurement precision
If traditional MPN method with separate sealing apparatus is used, then microbial quantification can be performed, but the device complexity increases
Solution Approach 1:
The device merges the sample distribution and sealing functions into a single integrated unit. The compartments are pre-formed with sealed lids that contain the sample distribution mechanism, eliminating the need for separate sealing apparatus and reducing overall device complexity while maintaining MPN quantification accuracy.
4Manufacturing precision
If automated instrument distribution is used, then sample distribution precision can be improved, but device complexity and cost increase
Solution Approach 1:
The device uses self-service capillary action to distribute samples automatically without requiring external automated instruments. The capillary channels and compartment design enable passive, precise sample distribution based on wicking principles, eliminating complex automated distribution systems while maintaining precision.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The device enables efficient and accurate detection and quantification of microorganisms with reduced operator intervention and improved accuracy by using capillary flow to distribute samples and a medium that indicates microbial presence through visible changes.
Implementation Method 1
Each capillary channel is adapted to transport liquefied sample from the sample-landing zone to the recessed compartment
Data Source
AI summary
There is provided a device for partitioning a liquefied sample into discrete volumes. The device includes a bottom member; a top member disposed adjacent the bottom member; and at least one channel member disposed between the top and bottom members. The at least one channel member is at least partially defined by the top and bottom members and has first and second end portions. The first end portion of the at least one channel has an opening to receive liquid and the second end portion of the at least one channel has a reaction compartment and a vent opening. Accordingly, when the liquefied sample is introduced to the first end portion, capillary action assists in causing the liquefied sample to travel from the first end portion to the second end portion and at least a portion of the liquefied sample is caused to remain in the reaction compartment.