Capillary Optical Filter for Multi-Target Signal Localization

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Solution Overview

Problem

Existing methods for simultaneously detecting multiple target substances, such as BIST™, face challenges in localizing signal light from probe beads, leading to interference and inaccurate measurements across a broad range of detection concentrations.

Innovation Solution

The use of an optical filter to selectively receive light emission from desired probe beads, reducing signal light diffusion by increasing the transparency of the container material and applying colorants to achieve a light transmittance of 70% or less in the wavelength band of emission.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If multiple probe beads are aligned in a capillary for simultaneous detection, then multi-item detection capability is improved, but signal light interference between adjacent probe beads increases

Engineering Contradiction:

Improvemulti-item detection capabilityVSAvoidsignal light interference

Core Design Contradiction:

Adaptability or versatilityVSMeasurement precision

Solution Approach 1:

The patent applies local quality by making different regions of the capillary have different light transmittance properties. Specifically, light-shielding portions are created at positions corresponding to probe beads that should not be detected, while light-transmissive portions are maintained at positions of probe beads to be detected. This spatial variation in optical properties allows simultaneous detection of multiple targets while blocking interference signals from adjacent probe beads.

Inventive Principle:

Principle #3Local quality

Solution Approach 2:

The capillary is segmented into distinct functional regions: light-transmissive portions for signal detection and light-shielding portions for interference suppression. This segmentation is achieved by forming light-shielding portions at specific locations within the capillary wall, creating a patterned optical structure that selectively transmits or blocks light based on the position of underlying probe beads.

Inventive Principle:

Principle #1Segmentation

2Measurement precision

If light-shielding portions are formed in the capillary to block interference signals, then signal localization is improved, but manufacturing complexity increases

Engineering Contradiction:

Improvesignal localizationVSAvoidmanufacturing complexity

Core Design Contradiction:

Measurement precisionVSDevice complexity

Solution Approach 1:

The patent employs color changes or optical property modifications in the capillary material to create light-shielding portions. By changing the optical absorption or transmittance characteristics of specific capillary regions (through coloring, doping, or material composition variation), the structure achieves selective light blocking without requiring complex mechanical or geometric modifications, thereby simplifying manufacturing while maintaining signal localization precision.

Inventive Principle:

Principle #32Color changes

3Adaptability or versatility

If the detection concentration range is extended to cover 10^3 dynamic range, then measurement versatility is improved, but signal light interference between probe beads increases

Engineering Contradiction:

Improvedetection concentration rangeVSAvoidsignal light interference

Core Design Contradiction:

Adaptability or versatilityVSMeasurement precision

Solution Approach 1:

Inventive Principle:

Principle #3Local quality

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

This approach allows for effective localization of signal light from specific probe beads, enhancing the accuracy of multi-item simultaneous detection and quantification by reducing interference and improving the peak-to-width ratio of emission signals.

Implementation Method 1

the light emission from a desired carrier is selectively received by measuring the emission from the carriers through an optical filter

Methodology Applied

Scientific EffectOptical filtering: Filter (optical)

Implementation Method 2

the optical filter has a light transmittance of 70% or less in the wavelength band of emission from the target of measurement

Methodology Applied

Scientific EffectLight absorption: Absorption (EM radiation)

Implementation Method 3

applying colorants to achieve a light transmittance of 70% or less in the wavelength band of emission

Methodology Applied

Scientific EffectLight absorption by colored materials: Absorption (EM radiation)

Data Source

PatentUS10288606B2Analysis method and analysis kit for simultaneously detecting or quantitating multiple types of target substances

Publication Date: 2019.05.14 UNIVERSAL BIO RESEARCH CO LTD

AI summary

The present invention provides an analysis method for simultaneously detecting or quantifying plural species of target substances by measuring the light emission from plural carriers within a single container from outside of the container, these carriers comprising a plurality of substances that are respectively immobilized thereon for capturing the plural species of target substances, wherein the light emission from a desired carrier is selectively received by measuring the emission from the carriers through an optical filter. The present invention also provides an analysis kit for simultaneously detecting or quantifying plural species of target substances by measuring the light emission from plural carriers within a single container from outside of the container, these carriers comprising a plurality of substances that are respectively immobilized thereon for capturing the plural species of target substances, which comprises the plural carriers and an optical filter for selectively receiving the emission from a desired carrier.