Capsicum Resistance Gene Stacking for TSWV Strain Control
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Solution Overview
Problem
Current pepper cultivars with the Tsw gene are susceptible to Tsw-resistance breaking strains like Ve427RB, which can cause systemic infections, and existing resistance is compromised by high temperatures and plant age, necessitating a new source of resistance against these strains.
Innovation Solution
Introducing a new resistance gene from wild Capsicum accession PA2638, which confers resistance to Ve427RB and can be stacked with the Tsw gene to provide broad-spectrum resistance against both wild-type and resistance-breaking TSWV strains.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If the Tsw resistance gene is introduced into pepper plants, then resistance to wild-type TSWV strains is improved, but susceptibility to Tsw-resistance breaking strains (e.g., Ve427RB) increases
Solution Approach 1:
The patent combines the Tsw resistance gene with a new resistance gene from wild Capsicum accession PA2638 into a single pepper plant variety. This gene stacking approach merges two different resistance mechanisms to achieve broad-spectrum resistance that protects against both wild-type TSWV strains and Tsw-resistance breaking strains simultaneously
Solution Approach 2:
The patent creates a composite genetic structure by integrating multiple resistance genes (Tsw and the new PA2638-derived gene) into the pepper plant genome. This composite genetic makeup provides layered defense mechanisms, where each gene targets different viral strains, resulting in comprehensive protection against diverse TSWV pathotypes
2Reliability
If the Tsw resistance gene is used in pepper plants, then protection against TSWV is achieved, but resistance effectiveness decreases under high temperatures and in older plants
Solution Approach 1:
The patent introduces a new resistance gene from wild Capsicum accession PA2638 that exhibits different physiological characteristics compared to the Tsw gene. This new gene maintains resistance effectiveness across varying environmental conditions including high temperatures and throughout different plant developmental stages, compensating for the temperature and age-related limitations of the Tsw gene
3Reliability
If commercial pepper cultivars are bred with the Tsw gene, then resistance to TSWV is improved, but the complexity of breeding programs increases due to the need to stack multiple resistance genes
Solution Approach 1:
The patent extracts a specific resistance gene from wild Capsicum accession PA2638 and isolates it as a distinct genetic element that can be independently transferred into commercial pepper cultivars. This extracted gene serves as a modular resistance component that can be added to existing Tsw-containing varieties through targeted breeding programs, simplifying the overall breeding approach compared to searching for multiple separate resistance genes
Data Source
AI summary
The present invention relates to the field of pepper breeding. Provided are plants which are resistant against a Tsw-resistance breaking pathotype of TSWV. Also provided are plant parts, seeds, cells and fruits thereof and methods to produce such plants.