Capture and Assist Probe PALSAR for Ultrasensitive Analyte Measurement
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Solution Overview
Problem
Conventional methods for measuring analytes such as target antibodies and nucleic acids are complex, costly, and require multiple antibodies, leading to high preparation costs and complicated performance management.
Innovation Solution
An ultrasensitive method using a capture probe and an assist probe in an improved PALSAR method, allowing for the hybridization of self-assembly probes to form a signal probe polymer, which is then used to detect and quantify target antibodies and nucleic acids.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional double antigen bridging immunoassay is used to measure anti-drug antibodies, then measurement capability is achieved, but preparation cost increases and operational complexity increases due to requiring multiple antibodies
Solution Approach 1:
The patent uses a capture probe that is a nucleic acid copy or representative of the drug molecule, rather than requiring multiple different antibodies. This single capture probe can capture various anti-drug antibodies, simplifying the reagent system while maintaining measurement capability
Solution Approach 2:
The capture probe serves multiple functions: it acts as a capture reagent, a signal amplification template, and can be used across different drug targets. This multi-functionality eliminates the need for separate capture antibodies for each drug, reducing overall complexity
2Measurement precision
If conventional PALSAR method is used for signal amplification, then sensitivity is improved, but reaction time increases and operational steps become more complex
Solution Approach 1:
The assist probe is pre-hybridized with the target nucleic acid in a preliminary step, creating a stable template before the honeycomb probes are added. This preliminary action optimizes the subsequent self-assembly reaction, reducing overall reaction time while maintaining sensitivity
Solution Approach 2:
The patent optimizes the dynamic aspects of the PALSAR reaction by adjusting incubation temperatures and times for each step. The two-stage process allows dynamic optimization where the first stage uses conditions optimal for assist probe hybridization, and the second stage uses conditions optimal for honeycomb probe self-assembly
3Reliability
If multiple drug antibodies are prepared for capture and tracer reagents, then measurement capability is achieved, but preparation cost and quality control complexity increase
Solution Approach 1:
Instead of preparing multiple different drug antibodies, the patent uses a nucleic acid-based capture probe that can be synthesized chemically. This synthetic approach is more cost-effective and easier to manufacture than producing multiple monoclonal antibodies, while maintaining reliable measurement capability
Solution Approach 2:
The nucleic acid capture probe is a simpler, cheaper alternative to protein-based antibodies. It can be synthesized at lower cost and does not require complex antibody production, purification, and validation processes, reducing both preparation cost and quality control complexity
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables simple and inexpensive measurement of analytes with improved sensitivity and reduced operational complexity.
Implementation Method 1
specifically hybridizable to a target nucleic acid
Implementation Method 2
a method using a pair of self-assembly probes composed of first and second oligonucleotides
Data Source
AI summary
Provided is an ultrasensitive method for measuring an anti-drug antibody that is simpler and less expensive than conventional methods. Provided is an ultrasensitive method for measuring an analyte using a capture probe and an assist probe and adopting an improved PALSAR method. By using the capture probe and the assist probe and adopting the improved PALSAR method in a double antigen bridging immunoassay, ultrasensitive measurement of an anti-drug antibody can be performed simply and inexpensively.