Capture Probe Panel for Simultaneous Rash Syndrome Pathogen Detection
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Solution Overview
Problem
Current diagnostic methods are inadequate for rapidly and accurately diagnosing multiple pathogens causing rash syndrome, particularly in the context of increasing infectious disease threats, and there is a need for a technological solution that enables simultaneous detection of viruses and bacteria associated with this syndrome.
Innovation Solution
A capture probe set comprising specific sequences (SEQ ID NO: 1 to SEQ ID NO: 3828) is developed for simultaneous detection of multiple pathogens, including Anaplasma phagocytophilum, Bacillus anthracis, and others, using next-generation sequencing (NGS) technology, with hybridization probes designed to capture key genetic regions of these pathogens.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If conventional diagnostic methods are used to identify pathogens, then diagnostic accuracy for single pathogens can be achieved, but simultaneous detection of multiple pathogens is not possible
Solution Approach 1:
The patent creates a universal capture probe set that can simultaneously detect multiple pathogens (viruses and bacteria) causing rash syndrome through a single NGS-based testing system. The probe set includes probes targeting different pathogens, enabling one system to perform multiple detection functions that would otherwise require separate diagnostic tests for each pathogen.
Solution Approach 2:
The patent combines multiple pathogen detection capabilities into a single integrated NGS testing system. By merging the detection of various viruses and bacteria into one unified assay using capture probes and next-generation sequencing, the system achieves simultaneous multi-pathogen detection without requiring separate diagnostic procedures for each pathogen.
2Measurement precision
If multiple separate tests are performed to detect different pathogens, then detection coverage is comprehensive, but diagnostic time and complexity increase
Solution Approach 1:
The patent merges multiple separate pathogen detection tests into a single NGS-based multi-pathogen panel. By combining the detection of various viruses and bacteria into one unified assay using capture probes and next-generation sequencing, the system achieves simultaneous multi-pathogen detection in a single workflow, dramatically reducing the time required compared to performing separate tests for each pathogen while maintaining comprehensive detection coverage.
3Adaptability or versatility
If NGS technology is implemented for multi-pathogen detection, then simultaneous detection capability is achieved, but development and implementation complexity increases
Solution Approach 1:
The patent segments the complex NGS-based multi-pathogen detection system into manageable components: a standardized capture probe set with probes targeting specific pathogens, a defined panel of rash syndrome-causing pathogens, and a structured analysis pipeline. This segmentation makes the complex NGS technology more implementable by breaking it down into modular elements that can be developed and validated systematically.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The capture probe set enhances diagnostic accuracy and efficiency by enabling simultaneous detection of multiple pathogens, improving sensitivity, specificity, and facilitating proactive responses to potential infectious disease outbreaks.
Implementation Method 1
hybridization probes designed to capture key genetic regions of these pathogens
Data Source
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AI summary
The present invention relates to a capture probe set for simultaneous detection of viruses and bacteria based on rash syndrome and use thereof