Cardiomyocyte Subset Identification via Cell Surface Marker Binding
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Solution Overview
Problem
Current methods lack an efficient way to distinguish and isolate cardiomyocyte subsets from heterogeneous cellular samples, which is crucial for cardiovascular disease research and therapy.
Innovation Solution
The method involves contacting a heterogeneous cellular sample with cell surface marker specific binding members, such as CD77, to differentiate cardiomyocytes based on binding or absence of binding, allowing for the separation and identification of cardiomyocyte subsets using flow cytometry.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional methods are used to analyze heterogeneous cellular samples, then general cell analysis can be performed, but specific cardiomyocyte subset identification and isolation cannot be achieved
Solution Approach 1:
The patent employs flow cytometry which detects cell surface markers through fluorescent labeling, enabling visual differentiation and identification of cardiomyocyte subsets based on their unique marker expression profiles. This allows precise identification of specific cardiomyocyte populations within heterogeneous samples.
Solution Approach 2:
The invention utilizes specific cell surface markers (such as CD77, CD172a, CD340) as distinguishing parameters to identify and isolate different cardiomyocyte subsets. By measuring and comparing marker expression levels, the method achieves precise subset identification without requiring complex procedural modifications.
2Productivity
If heterogeneous cellular samples are analyzed without specific markers, then sample processing is simpler, but cardiomyocyte subset separation and isolation are not achieved
Solution Approach 1:
The patent employs flow cytometry technology that can simultaneously detect multiple cell surface markers on different cell populations within a single heterogeneous sample. This multi-functional approach enables identification and isolation of various cardiomyocyte subsets (atrial, ventricular, conduction system) in one analytical process, significantly improving isolation efficiency.
Solution Approach 2:
The method uses specific binding members (antibodies) that replicate the specificity of natural cell surface marker interactions. These binding members serve as detectable copies or proxies for the actual cell surface markers, enabling indirect detection and isolation of cardiomyocyte subsets without directly manipulating the markers themselves.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach enables effective identification, characterization, and isolation of cardiomyocyte subsets, enhancing research and therapeutic applications for cardiovascular diseases by improving the accuracy and efficiency of cardiomyocyte identification and isolation.
Implementation Method 1
contacting the heterogeneous cellular sample with a cell surface marker specific binding member, and then distinguishing a cardiomyocyte subset of the cellular sample based on binding or absence of binding to the cell surface marker specific binding member
Data Source
AI summary
Methods are provided for distinguishing a cardiomyocyte subset in a heterogeneous cellular sample. Aspects of the methods include contacting the heterogeneous cellular sample with a cell surface marker specific binding member, and then distinguishing a cardiomyocyte subset of the cellular sample based on binding to the cell surface marker specific binding member. Also provided are devices, compositions and kits that find use in performing the subject methods.