Insoluble Carrier Amplification Reagent Layout for Rapid Diagnostics
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Solution Overview
Problem
Current immunochromatography methods face challenges in achieving normal amplification reactions while minimizing background signals, requiring complex setups and taking significant time, especially in rapid diagnostics like influenza testing.
Innovation Solution
A chromatography method involving the addition of a first amplification reagent upstream of the test sample on an insoluble carrier, with both developed in the same direction, and a second amplification reagent infiltrated in the thickness direction, to inhibit background signals and expedite the measurement process.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If amplification solutions are added to increase detection sensitivity, then detection sensitivity is improved, but background signals increase and test time extends
Solution Approach 1:
The patent applies preliminary action by pre-coating the insoluble carrier with a first amplification reagent before adding the test sample. This pre-prepared state allows the amplification reaction to begin immediately upon sample addition, eliminating the need for separate amplification solution addition steps and reducing overall test time while maintaining high detection sensitivity
Solution Approach 2:
The patent segments the amplification process into two distinct reagents: a first amplification reagent pre-coated on the carrier and a second amplification reagent added subsequently. This segmentation allows the amplification reaction to proceed in stages, improving detection sensitivity through dual-reagent amplification while controlling background signals and reducing total test time compared to single-step amplification methods
2Measurement precision
If amplification solutions are added to improve detection sensitivity, then detection sensitivity is improved, but the operation becomes complex
Solution Approach 1:
The patent merges the first amplification reagent directly onto the insoluble carrier, combining multiple functions (carrier support and amplification reagent delivery) into a single integrated component. This merging eliminates the need for separate amplification solution addition steps, simplifying the operation while maintaining high detection sensitivity through the combined amplification effect
Solution Approach 2:
By pre-coating the carrier with the first amplification reagent, the patent eliminates the need for operators to manually add amplification solutions during the test procedure. This preliminary preparation simplifies the operation to basic steps (sample addition and result reading) while achieving enhanced detection sensitivity through the pre-positioned amplification reagent
3Productivity
If amplification solutions are added to achieve normal amplification reactions, then amplification reaction efficiency is improved, but background signals increase
Solution Approach 1:
The patent segments the amplification reagents into two distinct components added at different times: the first amplification reagent pre-coated on the carrier and the second amplification reagent added after sample incubation. This segmentation allows the amplification reaction to proceed efficiently in stages, improving productivity while controlling background signals by avoiding premature introduction of all amplification components
Solution Approach 2:
The first amplification reagent is pre-coated on the carrier in a controlled manner, creating a localized amplification environment that will only become active when the test sample is added. This preliminary positioning ensures high amplification reaction efficiency when needed, while preventing background signal generation during storage and preparation phases when the reagents are not yet activated
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach enables normal amplification reactions, reduces background signals, simplifies the operation, and allows for rapid and convenient measurement, particularly beneficial for speedy diagnostics.
Implementation Method 1
a step of forming a composite with a test substance and a labeling substance containing a metal modified by a first binding substance of the test substance and then developing the composite on an insoluble carrier; a step of amplifying the captured labeling substance using a first amplification reagent and a second amplification reagent
Implementation Method 2
the second amplification reagent is infiltrated into the insoluble carrier in the thickness direction of the insoluble carrier
Data Source
AI summary
The chromatography method includes a step of forming a composite with a test substance and a labeling substance containing a metal modified by a first binding substance of the test substance and then developing the composite on an insoluble carrier; a step of capturing the test substance and the labeling substance in a detection site on the insoluble carrier including a second binding substance of the test substance or a substance having a binding property to the first binding substance of the test substance; and a step of amplifying the captured labeling substance using a first amplification reagent and a second amplification reagent to detect the test substance.