Carrier Peptide Fragment RKLAVYWSTYKRSR for Broad Cell Delivery
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Solution Overview
Problem
Existing methods for transferring foreign substances into eukaryotic cells, such as polypeptides and biologically active substances, face variability in cell penetrating efficiency due to the combination of amino acid sequences and foreign substances, necessitating improved techniques for targeted delivery.
Innovation Solution
A novel carrier peptide fragment with the amino acid sequence RKLAVYWSTYKRSR (SEQ ID No.: 1) is developed, which exhibits cell membrane permeability, allowing for the efficient transfer of foreign substances like polypeptides, nucleic acids, and drugs into eukaryotic cells by bonding them to the N- or C-terminus of this peptide.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional CPPs are used to transfer foreign substances into cells, then cell penetrating function is achieved, but cell penetrating efficiency varies depending on the combination of amino acid sequence and foreign substance
Solution Approach 1:
The patent develops a universal carrier peptide fragment (amino acid sequence: RKLAVYWSTYKRSR) that can efficiently transfer multiple types of foreign substances including proteins, peptides, nucleic acids, and chemical substances into eukaryotic cells. This single peptide sequence demonstrates broad applicability across different cargo types, eliminating the need to select different CPPs for different foreign substances.
2Reliability
If foreign substances are transferred into eukaryotic cells to change cell characteristics or improve function, then therapeutic effect is achieved, but delivery efficiency varies with substance-CPP combination
Solution Approach 1:
The carrier peptide fragment serves as a universal delivery vehicle for diverse therapeutic agents including proteins, peptides, nucleic acids, and chemical substances. The single peptide sequence RKLAVYWSTYKRSR can be coupled with various foreign substances through standard chemical coupling methods, providing a simplified platform that reduces the complexity of selecting and optimizing different CPP-FS combinations for different therapeutic applications.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The carrier peptide fragment effectively transfers foreign substances into eukaryotic cells, enhancing the efficiency and stability of delivery, particularly for polypeptides, nucleic acids, and drugs, with applications in stem cell differentiation, antitumor activity, and antivirus activity.
Implementation Method 1
This carrier peptide fragment has the cell membrane permeability, and can transfer a foreign substance from outside a cell into the cell
Data Source
Figure 1
AI summary
The present disclosure provides a novel carrier peptide fragment with cell membrane permeability. The carrier peptide fragment disclosed herein includes an amino acid sequence: RKLAVYWSTYKRSR (SEQ ID No.: 1).