Carrier Peptide Fragment RKKRNSKRLTRKK for Cell Permeability
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Solution Overview
Problem
Existing methods for transferring foreign substances into eukaryotic cells are limited by variable cell membrane permeability, which restricts the range of medical applications.
Innovation Solution
A novel carrier peptide fragment with a 13-amino acid sequence (RKKRNSKRLTRKK) is developed, which exhibits superior cell membrane permeability, enabling efficient transfer of target foreign substances into the cytoplasm of eukaryotic cells.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If a carrier peptide fragment with known NoLS sequence is used, then cell membrane permeability is achieved, but the efficiency varies depending on cell species limiting medical treatment range
Solution Approach 1:
The patent modifies the amino acid sequence parameters of the carrier peptide fragment, specifically incorporating D-amino acids (D-arginine and D-lysine) at positions 1 and 2 of the sequence. This parameter change in the peptide structure significantly improves cell membrane permeability efficiency across different cell species, thereby expanding the adaptability and range of medical treatment applications.
2Reliability
If conventional carrier peptides are used, then foreign substance transfer is possible, but the cell membrane permeability varies in efficiency
Solution Approach 1:
The patent changes the chemical parameters of the carrier peptide by incorporating D-amino acids, which alters the peptide's interaction with cell membranes. This parameter modification achieves both high reliability in cell membrane permeability and high productivity in foreign substance transfer efficiency, resolving the contradiction between these two factors.
Data Source
Figure 1
AI summary
The present disclosure provides a carrier peptide fragment with the cell membrane permeability that can transfer a target foreign substance from outside a eukaryotic cell into at least a cytoplasm of the cell. The carrier peptide fragment disclosed herein includes an amino acid sequence: RKKRNSKRLTRKK.