Carrier Peptide Fragment Chirality for Cell Membrane Permeability
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Solution Overview
Problem
Existing carrier peptides do not exhibit sufficient cell membrane permeability for efficient transfer of foreign substances into eukaryotic cells.
Innovation Solution
A carrier peptide fragment with an amino acid sequence of KKRTLRKSNRKKR, where the lysine residue at the 12th position is in the D-form, enhancing cell membrane permeability, is used to transfer foreign substances such as polypeptides, nucleic acids, and drugs into eukaryotic cells.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If a conventional carrier peptide fragment (SEQ ID No.: 2) is used, then the construct can be transferred into eukaryotic cells, but the cell membrane permeability is insufficient
Solution Approach 1:
The patent applies parameter changes by inverting the chirality of the lysine residue at the 12th position from L-form to D-form in the carrier peptide fragment. This stereochemical parameter change fundamentally alters the interaction with the cell membrane, transforming the peptide from having insufficient permeability to achieving remarkable cell membrane permeability and efficient transfer of foreign substances into eukaryotic cells
Data Source
AI summary
The present disclosure provides a carrier peptide fragment with excellent cell membrane permeability. The carrier peptide fragment disclosed herein includes the following amino acid sequence set forth in SEQ ID No.: 1: KKRTLRKSNRKKR, and a lysine residue at a 12th position from an N-terminus of the amino acid sequence is a D-form, and the other amino acid residues are L-forms.