Cartridge-Based Rapid Test Analyzer Using MAC and Optical Sensing
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Solution Overview
Problem
Current COVID-19 detection methods, such as RT-PCR, are time-consuming, require skilled personnel and expensive equipment, and are not suitable for rapid screening at points of entry or in resource-limited settings, necessitating a need for rapid, portable, and cost-effective testing solutions.
Innovation Solution
A rapid test device utilizing Microscale Affinity Chromatography (MAC) and optical molecular sensing technology, which includes a cartridge with micromagnetic particles and fluorescent-labeled reagents for rapid detection of COVID-19 antigens and antibodies in saliva samples, providing results in minutes without the need for extensive equipment or trained personnel.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If RT-PCR is used for detection, then measurement precision is improved, but testing time increases and device complexity increases
Solution Approach 1:
The invention extracts the essential detection function from complex RT-PCR laboratories and concentrates it into a portable cartridge-based device. The cartridge contains pre-loaded reagents, magnetic beads for sample preparation, and integrated detection components, eliminating the need for lengthy laboratory procedures while maintaining detection accuracy.
Solution Approach 2:
The invention replaces the complex mechanical and chemical amplification steps of RT-PCR with a simplified system using magnetic bead-based separation and fluorescent detection. This substitution reduces testing time from hours to minutes while preserving measurement precision through optimized binding and detection mechanisms.
2Measurement precision
If RT-PCR equipment is used, then measurement precision is improved, but device complexity and cost increase
Solution Approach 1:
The invention segments the detection system into a self-contained cartridge that can be used independently without complex supporting equipment. Each cartridge is a discrete unit containing all necessary reagents and components, transforming a complex centralized system into simple, portable units that maintain precision while reducing overall device complexity.
Solution Approach 2:
The invention employs disposable cartridges that eliminate the need for expensive, complex reusable equipment. Each single-use cartridge contains pre-prepared reagents and magnetic beads, reducing the need for costly laboratory infrastructure while maintaining detection accuracy through optimized cartridge design.
3Productivity
If rapid screening is implemented, then productivity is improved, but measurement precision may worsen
Solution Approach 1:
The invention performs preliminary actions by pre-loading the cartridge with optimized reagent concentrations, pre-coated magnetic beads, and pre-configured detection components. This preliminary preparation enables rapid testing without sacrificing precision, as all critical parameters are optimized in advance during cartridge manufacturing rather than during the testing process.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables rapid, accurate detection of COVID-19 antigens and antibodies, reducing testing time to minutes, increasing accessibility for point-of-care testing, and facilitating the assessment of immunity, thereby aiding in controlling the pandemic and potential future outbreaks.
Implementation Method 1
the first chamber pre-filled with micromagnetic particles treated so as to bind to the target substance
Implementation Method 2
a first reservoir pre-filled with at least one reagent labeled with at least one fluorescent compound, the reagent adapted to bind to micromagnetic particles that are bound to the target substance
Implementation Method 3
an apparatus comprising a light source disposed so as to excite the least one fluorescent compound with a light
Data Source
AI summary
Embodiments may include a rapid test device that provide rapid detection of substances, including those involved in pathogen infection, for example, using Microscale Affinity Chromatography (MAC), indirect ELISA, and optical molecular sensing technology. For example, in an embodiment, a system may comprise a cartridge comprising a first chamber configured to receive a test sample including a target substance, the first chamber pre-filled with micromagnetic particles treated so as to bind to the target substance, and a first reservoir pre-filled with at least one reagent labeled with at least one fluorescent compound, the reagent adapted to bind to micromagnetic particles that are bound to the target substance, and an apparatus comprising a light source disposed so as to excite the least one fluorescent compound with a light and an optical sensor disposed so as to detect an emitted spectrum of light from the excited least one fluorescent compound.