Cartridge-Based Sequencing for Automated Sample-to-Result Workflows

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Solution Overview

Problem

Current nucleic acid sequencing workflows are tedious, time-consuming, costly, and require skilled personnel, with low sample input capacity and long turnaround times, limiting the practical application of next-generation sequencing.

Innovation Solution

A fully automated system comprising a cartridge and benchtop instrument performs sample preparation, library preparation, and sequencing without user intervention, using a semiconductor chip for detection and nucleic acid cluster generation, capable of processing a wide range of sample types and amounts within a few hours.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Measurement precision

If manual sequencing workflows are used with multiple instruments and skilled personnel, then sequencing accuracy can be maintained, but the process becomes tedious, time-consuming, and complex

Engineering Contradiction:

Improvesequencing accuracyVSAvoidworkflow complexity

Core Design Contradiction:

Measurement precisionVSDevice complexity

Solution Approach 1:

The patent combines sample preparation, library preparation, and sequencing operations into a single integrated cartridge system. This merging of previously separate steps and instruments into one unified device reduces workflow complexity while maintaining sequencing accuracy through automated, standardized processes.

Inventive Principle:

Principle #5Merging (Combining)

Solution Approach 2:

The sequencing cartridge is designed as a multi-functional device that can perform multiple operations (sample preparation, library preparation, sequencing) within a single instrument. This universal design eliminates the need for multiple specialized instruments and skilled personnel interventions at different stages.

Inventive Principle:

Principle #6Universality (Multi-functionality)

2Measurement precision

If traditional sequencing workflows are used, then comprehensive sample analysis is possible, but the turnaround time from sample to result is several hours to several days

Engineering Contradiction:

Improvesample analysis completenessVSAvoidturnaround time

Core Design Contradiction:

Measurement precisionVSLoss of time

Solution Approach 1:

The automated cartridge system enables continuous processing where sample preparation, library preparation, and sequencing occur in an uninterrupted sequence without manual intervention between steps. This continuous workflow eliminates idle time and reduces the overall turnaround time from sample to result.

Inventive Principle:

Principle #20Continuity of useful action

Solution Approach 2:

The cartridge pre-loads all necessary reagents, primers, and enzymes required for the complete sequencing workflow before the sample is introduced. This preliminary preparation of all components allows the process to proceed immediately without waiting for reagent additions or instrument reconfigurations.

Inventive Principle:

Principle #10Preliminary action

3Adaptability or versatility

If manual sequencing processes are used, then flexible sample handling is possible, but the maximum allowable sample input is low and requires highly skilled personnel

Engineering Contradiction:

Improvesample handling flexibilityVSAvoidoperational simplicity

Core Design Contradiction:

Adaptability or versatilityVSEase of operation

Solution Approach 1:

The cartridge system is designed to automatically handle sample processing without requiring skilled personnel for manual operations. The system self-manages reagent delivery, sample preparation, and sequencing operations, making the process accessible to users without specialized training while maintaining flexibility for different sample types.

Inventive Principle:

Principle #25Self-service

4Measurement precision

If multiple instruments and ancillary components are used for sequencing, then comprehensive sequencing capability is achieved, but the instrument footprint and handling complexity increase

Engineering Contradiction:

Improvesequencing capabilityVSAvoidinstrument footprint

Core Design Contradiction:

Measurement precisionVSArea of stationary object

Solution Approach 1:

The patent integrates sample preparation, library preparation, and sequencing functions into a single compact cartridge that fits within one benchtop instrument. This consolidation eliminates the need for multiple separate instruments and ancillary components, significantly reducing the overall instrument footprint while maintaining comprehensive sequencing capability.

Inventive Principle:

Principle #5Merging (Combining)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The system provides rapid, sensitive, and accurate sequencing results with minimal human intervention, accommodating various assays and sample types, and achieving high sensitivity for low levels of nucleic acid presence, while reducing the instrument's footprint and handling complexity.

Implementation Method 1

use of a semiconductor chip for detection

Methodology Applied

Scientific EffectSemiconductor detection:

Implementation Method 2

methods for the generation of nucleic acid clusters on a surface, including the surface of a semi-conductor chip

Methodology Applied

Scientific EffectNucleic acid amplification:

Data Source

PatentUS20250269368A1Method and system comprising a cartridge for sequencing target polynucleotides

Publication Date: 2025.08.28 DNAE GROUP HOLDINGS LIMITED

AI summary

Described are methods of identifying a target polynucleotide sequence from a sample using a cartridge-based system and methods of forming an array of amplified nucleic acid molecules on a solid support.