Casein Peptide Teat Seal for Bovine Mastitis Prevention

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Solution Overview

Problem

Current treatments for bovine mastitis, such as antimicrobial agents, often lead to bacterial resistance and do not effectively address the underlying issues of mammary gland infections, and there is a need for improved measures to prevent and treat mastitis in dairy cattle.

Innovation Solution

A therapeutic combination comprising casein proteins or casein-derived peptides, including β-casein, αS1-casein, αS2-casein, or K-casein, administered with a teat seal composition forming a physical barrier, which can be a dip, hydrogel, or biofilm, containing non-toxic heavy metal salts like bismuth, titanium, or zinc, to prevent and treat mastitis.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If antimicrobial agents are used to treat mastitis, then bacterial infection is addressed, but bacterial resistance develops and underlying issues are not resolved

Engineering Contradiction:
Improveeffectiveness of mastitis treatmentVSAvoidbacterial resistance
Core Design Contradiction:
ReliabilityVSObject-generated harmful factors

Solution Approach 1:

The patent extracts and removes harmful antimicrobial agents from the treatment approach, replacing them with casein-derived peptides and teat seal compositions that physically and biologically prevent bacterial infection without promoting resistance. The solution takes out the problematic element (antimicrobials) and replaces it with natural, non-resistant-inducing alternatives.

Inventive Principle:
Principle #2Taking out (Extraction)

Solution Approach 2:

The teat seal composition is applied in advance to create a physical barrier before bacterial infection occurs. The casein-derived peptides are administered preliminarily to modulate the mammary gland environment, preventing infection rather than treating it after onset, thereby eliminating the need for antimicrobials that cause resistance.

Inventive Principle:
Principle #10Preliminary action

2Reliability

If teat seal composition with heavy metal salts is used, then physical barrier is formed to prevent infection, but metal residues may accumulate in milk

Engineering Contradiction:
Improveprevention of mastitisVSAvoidmetal residues in milk
Core Design Contradiction:
ReliabilityVSObject-affected harmful factors

Solution Approach 1:

The patent changes the concentration and composition parameters of the teat seal formulation, using low concentrations of non-toxic heavy metal salts (bismuth, titanium, zinc) combined with casein-derived peptides. This parameter modification reduces metal residue accumulation while maintaining the physical barrier function for mastitis prevention.

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

Casein-derived peptides act as an intermediary substance that facilitates the formation and stability of the teat seal barrier while controlling metal salt deposition. The peptides mediate between the heavy metal salts and the mammary gland tissue, ensuring barrier formation without excessive metal accumulation in milk.

Inventive Principle:
Principle #24Intermediary (Mediator)

3Reliability

If casein proteins or peptides are administered, then mammary gland function is improved, but formulation complexity increases

Engineering Contradiction:
Improvemammary gland healthVSAvoidtherapeutic combination formulation
Core Design Contradiction:
ReliabilityVSDevice complexity

Solution Approach 1:

The patent merges casein-derived peptides and teat seal compositions into a unified therapeutic combination that can be administered together. This combination approach consolidates multiple functions (mammary gland modulation and physical barrier formation) into a single treatment protocol, reducing overall complexity despite the multi-component nature of the formulation.

Inventive Principle:
Principle #5Merging (Combining)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The combination effectively reduces mastitis symptoms, improves milk hygiene, and increases milk yield without worsening milk quality, while also reducing metal residues in milk, thus addressing the limitations of existing treatments.

Implementation Method 1

the teat seal composition forms a physical barrier on a teat surface, in a teat canal, or in a teat cistern of an animal

Methodology Applied
Scientific EffectPhysical barrier: Physical Containment

Implementation Method 2

the teat seal composition comprises a heavy metal, or salt thereof. In certain embodiments, the teat seal composition comprises a non-toxic heavy metal salt. In certain embodiments, the non-toxic heavy metal salt is selected from the group consisting of bismuth salt, titanium salt, zinc salt, barium salt, and any combination thereof

Methodology Applied
Scientific EffectAntimicrobial activity:

Implementation Method 3

a composition comprising at least one casein protein or casein-derived peptide

Methodology Applied
Scientific EffectImmune modulation:

Implementation Method 4

Casein-derived peptides were previously reported in the treatment of mammary gland infection

Methodology Applied
Scientific EffectAnti-inflammatory activity:

Data Source

PatentUS20240207352A1Compositions and methods for modulating mammary disorders and conditions
Publication Date: 2024.06.27 MILEUTIS

AI summary

The present disclosure provides compositions and methods for modulating various mammary' disorders and conditions. The disclosure further provides a therapeutic combination of a composition of at least one casein protein or casein-derived peptide; and a teat seal composition.