CasMG RNA-Guided Nucleases for Diverse Genome Editing
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Solution Overview
Problem
Current CRISPR/Cas systems for genomic modifications and diagnostics lack alternative nucleases with diverse properties to optimize performance across various genome editing and diagnostic applications.
Innovation Solution
Development of RNA-guided endonuclease polypeptides and DNA binding proteins, referred to as CasMG proteins, along with their corresponding guide RNAs, which provide sequence specificity and are useful in various applications, including genome editing and diagnostics.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If conventional CRISPR/Cas systems are used for genomic modifications, then genome editing capability is achieved, but lack of alternative nucleases with diverse properties limits optimization for specific applications
Solution Approach 1:
The patent modifies the Cas protein by changing parameters such as nucleotide binding specificity, cleavage activity, and guide RNA compatibility to create variants (CasMG1-CasMG10) with different properties optimized for specific applications including genome editing, base editing, and diagnostic assays
2Productivity
If a single CRISPR/Cas system is used for multiple applications, then system simplicity is maintained, but performance optimization for different genomic contexts is limited
Solution Approach 1:
The patent segments the CRISPR/Cas system into multiple specialized nuclease variants (CasMG1-CasMG10), each optimized for specific functions such as double-strand breaks, single-strand nicks, base editing, or diagnostic applications, allowing selection of the appropriate tool for each genomic context
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The CasMG proteins and guide RNAs enable site-specific activity and versatility in genome editing and diagnostic applications, addressing the need for alternative nucleases with optimal performance across different genomic contexts.
Implementation Method 1
guide RNAs that bind to and provide sequence specificity to the CasMG proteins
Data Source
AI summary
Compositions and methods related to Cas proteins, nucleic acids encoding the Cas proteins, and modified host cells comprising the Cas proteins and/or encoding nucleic acids are disclosed. Cas proteins are useful in a variety of applications. Cas proteins bind guide RNAs that in turn provide functional specificity to the Cas proteins, nucleic acids encoding the Cas guide RNAs, and modified host cells comprising the Cas guide RNAs and/or encoding nucleic acids. The Cas polypeptides and corresponding guide RNAs can be used in a variety of applications.