CasP6 RNA-Guided Nucleases and Guide RNAs for Targeted Editing
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Solution Overview
Problem
There is a need for alternative CRISPR/Cas nucleases with different properties to optimize performance in genome modification and diagnostic applications.
Innovation Solution
Development of RNA-guided endonuclease polypeptides (CasP6 proteins) and guide RNAs (CasP6 guide RNAs) that provide sequence specificity and functionality for targeted nucleic acid modification and detection.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If conventional CRISPR/Cas nucleases are used, then genome modification capability is achieved, but performance optimization is limited due to lack of alternative nucleases with different properties
Solution Approach 1:
The patent applies universality by developing a new CasP6 nuclease that can perform genome modification functions similar to existing CRISPR/Cas nucleases while offering alternative properties for optimization. The CasP6 protein serves as a universal tool that expands the functionality available for genome editing applications.
2Measurement precision
If RNA-based masking construct is used in detection system, then detection capability is improved, but system complexity increases due to additional components
Solution Approach 1:
The patent employs an RNA-based masking construct as an intermediary component in the detection system. This masking RNA acts as a mediator that enhances detection capability by providing additional specificity and control mechanisms, while its modular nature allows it to be integrated without excessively increasing overall system complexity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The CasP6 proteins and guide RNAs enable precise and efficient genome editing and nucleic acid detection, offering improved performance in various applications.
Implementation Method 1
RNA-guided endonuclease polypeptides, referred to herein as 'CasP6' polypeptides
Implementation Method 2
one or more CasP6 guide RNAs each comprising a guide sequence that can hybridize to RNA molecules having corresponding target sequences
Implementation Method 3
the CasP6 comprises endonuclease enzyme activity
Data Source
AI summary
Compositions and methods related to Cas proteins, nucleic acids encoding the Cas proteins, and modified host cells comprising the Cas proteins and/or encoding nucleic acids are disclosed. Cas proteins are useful in a variety of applications. Cas proteins bind guide RNAs that in turn provide functional specificity to the Cas proteins, nucleic acids encoding the Cas guide RNAs, and modified host cells comprising the Cas guide RNAs and/or encoding nucleic acids. The Cas polypeptides and corresponding guide RNAs can be used in a variety of applications.