Electrophoretic Collagen Deposition on Cathode
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Solution Overview
Problem
Existing methods for preparing collagen materials, such as solution casting and electrophoretic deposition (EDP), face challenges including the difficulty in producing heteromorphic structures, long preparation times, and issues with uniformity and density of the collagen material.
Innovation Solution
A novel preparation method for a collagen material stripped from a cathode, which involves using acetic acid to adjust the pH of the collagen solution to its isoelectric point, and conducting electrophoretic deposition (EDP) with hydrogen peroxide, allowing for rapid and uniform deposition of collagen on the cathode.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of manufacture
If solution casting method is used to prepare collagen material, then the process is simple, but the preparation time is long (several hours or even a night) and the collagen fibers are disorderly arranged with low density
Solution Approach 1:
The patent replaces the passive mechanical diffusion process of solution casting with an active electrophoretic deposition process driven by electric fields. Collagen molecules are driven by electrophoresis under an electric field to migrate toward the cathode, where they deposit and assemble into ordered structures, dramatically reducing preparation time from hours to minutes while maintaining process simplicity
Solution Approach 2:
The patent changes key process parameters by introducing electric field strength, voltage, and current density as controllable variables. By adjusting these electrical parameters along with pH and temperature, the deposition rate and collagen assembly structure can be precisely controlled, enabling rapid preparation of high-density, ordered collagen materials
2Productivity
If electrophoretic deposition (EDP) technology is used to prepare collagen material, then the preparation time is reduced (30-60 min) and the collagen arrangement density is improved, but the isoelectric point region is located at a specified position in the electrolyte making material acquisition and shaping inconvenient
Solution Approach 1:
The patent inverts the traditional EDP approach by using the cathode electrode itself as the collection substrate for collagen material. Instead of allowing collagen to precipitate at the isoelectric point in the bulk electrolyte, the electric field drives collagen molecules directly to the cathode where they deposit and can be easily acquired by simply removing the electrode. This enables convenient shaping by designing electrodes with desired geometries
Solution Approach 2:
The patent introduces the cathode electrode as an intermediary substrate that mediates collagen deposition. The electrode surface serves as a temporary platform for collagen assembly, allowing controlled deposition and easy subsequent removal of the collagen material, thereby solving the inconvenience of material acquisition and shaping in traditional EDP methods
3Ease of manufacture
If pulsed EDP technology is used to acquire collagen material on electrode, then hydrogen bubbles are reduced to facilitate deposition, but the organic solvent causes structural change of collagen and the material structure uniformity is difficult to control
Solution Approach 1:
The patent changes the chemical environment parameters by using acetic acid to adjust pH to the isoelectric point of collagen, eliminating the need for organic solvents like ethanol. This maintains collagen structural integrity while still facilitating deposition. The uniformity of material structure is controlled through precise adjustment of electrical parameters (voltage, current density) and chemical parameters (pH, temperature) throughout the electrolyte volume
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method enables the rapid preparation of collagen materials with uniform and dense structures, transparent appearance, and excellent mechanical and optical properties, while also allowing for the recycling of the collagen material.
Implementation Method 1
An electric field is applied to an acidic solution of collagen to drive the electrophoretic migration of collagen molecules to a cathode region
Implementation Method 2
Because an electrochemical reaction (usually a water electrolysis reaction) at a cathode can increase a pH of a solution near the electrode, collagen swimming to a collagen isoelectric point region will be precipitated
Data Source
AI summary
A preparation method of a collagen material stripped from an electrode and a use of the collagen material are provided. The preparation method of a collagen material stripped from an electrode includes the following step: subjecting a collagen solution including hydrogen peroxide and/or acetic acid to electro-deposition (EDP) to obtain the collagen material on the electrode. The preparation method can lead to a collagen material directly on a surface of an electrode.