Cation Exchange Chromatography for Allergen Removal
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Solution Overview
Problem
The molecular origin of allergy in mammals remains unclear, with known allergens often being harmless to most individuals, and existing methods fail to effectively reduce allergenicity in food and pharmaceutical compositions.
Innovation Solution
The method involves treating compositions using cation exchange and/or affinity chromatography to remove cationic proteins resulting from transcription infidelity (TI) events, which are enriched in basic amino acids and have a modified C-terminal end, thereby reducing allergenicity by removing at least 50% of proteins with an isoelectric point above 7.4.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Object-affected harmful factors
If conventional methods are used to process food and pharmaceutical compositions, then the compositions retain their full protein content and nutritional/pharmaceutical value, but they maintain allergenicity due to the presence of cationic proteins resulting from transcription infidelity
Solution Approach 1:
The patent applies extraction by removing cationic proteins from compositions through cation exchange chromatography. The method specifically extracts proteins with pI > 7.4 that result from transcription infidelity, separating them from the bulk protein content while retaining nutritional and pharmaceutical components
Solution Approach 2:
The patent utilizes parameter changes by exploiting the isoelectric point (pI) difference between cationic allergenic proteins and other proteins. By adjusting pH conditions during cation exchange chromatography, the method selectively binds and removes proteins with pI > 7.4, changing the electrical charge state to achieve separation
2Object-affected harmful factors
If cation exchange chromatography is used to remove cationic proteins, then allergenicity is reduced by removing at least 50% of proteins with isoelectric point above 7.4, but the process complexity and manufacturing steps increase
Solution Approach 1:
The method changes pH parameters to control protein charge states, enabling selective binding of cationic proteins to the cation exchange resin. By adjusting pH below the isoelectric point of target proteins, they acquire positive charge and bind to the negatively charged resin, while other proteins pass through
Solution Approach 2:
The cation exchange resin acts as an intermediary medium that facilitates selective removal of cationic proteins. The resin provides a charged surface that temporarily binds target proteins, allowing separation without direct manipulation of the protein molecules themselves
3Object-affected harmful factors
If affinity chromatography is used to remove cationic proteins, then allergenicity is reduced with high specificity, but the manufacturing cost and process complexity increase
Solution Approach 1:
Affinity ligands are used as intermediaries that specifically recognize and bind to cationic proteins resulting from transcription infidelity. These ligands are immobilized on chromatography matrices, creating a selective capture system that removes allergens with high specificity
Solution Approach 2:
The affinity chromatography method extracts specific cationic allergenic proteins from compositions by exploiting unique molecular recognition interactions. The targeted proteins are selectively bound and removed, leaving the majority of protein content intact
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach significantly reduces allergenicity by eliminating proteins that trigger IgE production, making compositions hypoallergenic and less immunogenic, as demonstrated by the removal of TI gap proteins from milk and peanut products, which significantly decreases IgE induction in murine models.
Implementation Method 1
treating the composition by cation exchange and/or affinity chromatography to remove cationic proteins
Implementation Method 2
treating the composition by cation exchange and/or affinity chromatography to remove cationic proteins
Data Source
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AI summary
The present invention relates to compositions and methods for modulating or detecting allergy in a subject. The invention may be used to reduce allergenicity of compositions, such as food products, or to stimulate immunogenicity of products, such as vaccines. The invention may be used in any mammal such as human.