Cationic Graft Polymer Biomolecule Separation
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Solution Overview
Problem
Existing methods for biomolecule separation and concentration, such as ion exchange chromatography, often damage the target biomolecules due to the use of surfactants and high salt concentrations, making it difficult to maintain their three-dimensional structure and viability during the separation process.
Innovation Solution
A method using a cationic graft polymer, specifically a polyamine graft polymer obtained by polymerizing a polyamine derivative with a compound having an epoxy group and an ethylenically unsaturated monomer, which binds to the target biomolecule under basic conditions, allowing for efficient separation and concentration while minimizing damage, and is used in a kit that includes metal ion salts and acidic or chelating agents for elution and washing.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If ion exchange chromatography is used to separate and concentrate biomolecules, then separation efficiency is improved, but the target biomolecules are damaged due to surfactants and high salt concentrations
Solution Approach 1:
The invention changes the chemical parameters of the separation medium by using a cationic graft polymer in basic conditions instead of traditional ion exchange resins requiring high salt and surfactant conditions. This parameter change allows effective separation while preserving biomolecule integrity, directly resolving the contradiction between separation efficiency and biomolecule damage.
Solution Approach 2:
The cationic graft polymer acts as an intermediary substance that mediates the separation process. It provides a gentle alternative to harsh ion exchange conditions, enabling effective separation through electrostatic interactions in basic pH without requiring damaging surfactants or high salt concentrations, thus protecting biomolecules while maintaining separation efficiency.
2Quantity of substance
If traditional ion exchange methods are used, then biomolecule separation is achieved, but the three-dimensional structure and viability of the target are lost
Solution Approach 1:
By changing the pH parameter to basic conditions and using a cationic graft polymer instead of traditional ion exchange resins, the invention achieves biomolecule separation while maintaining the three-dimensional structure and viability. The basic pH conditions and cationic polymer interactions are gentler on protein structures compared to high salt and surfactant conditions.
3Object-affected harmful factors
If cationic graft polymer is used for separation, then biomolecule integrity is maintained, but the method complexity increases due to polymer synthesis requirements
Solution Approach 1:
The cationic graft polymer can be used as a disposable reagent in the separation process. The polymer is prepared in advance through a one-time synthesis process, then used for multiple separations without requiring complex regeneration or maintenance procedures. This approach reduces operational complexity while maintaining the gentle separation conditions that preserve biomolecule integrity.
Data Source
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AI summary
The purpose of the present invention is to provide a method for separating and concentrating a target substance as an alternative to existing cationic polymers, and a kit for implementing this method.