Sequential CBD Glycoside Desorption for High-Purity Separation
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Solution Overview
Problem
Existing technologies lack effective methods for efficiently separating and purifying cannabinoid glycosides, particularly CBD-glycosides, from complex mixtures, which are hindered by their hydrophobic nature and limited solubility, impacting their therapeutic use and industrial scalability.
Innovation Solution
A novel system and method utilizing solubility and lipophilic properties of CBD-glycosides for desorption from resins, followed by liquid/liquid extraction and crystallization, to separate and purify CBD, CBD1G, and CBD2G, achieving high purity and yield.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If fermentation method is used to generate water-soluble cannabinoid glycosides, then solubility is improved, but product capture and purification becomes difficult
Solution Approach 1:
The patent segments the complex mixture of cannabinoid glycosides into individual components through sequential desorption using different solvents. Each solvent selectively elutes specific glycoside species (monoglycosides, diglycosides, triglycosides) from the resin, enabling separate purification of each component despite their similar chemical structures and water solubility.
Solution Approach 2:
The patent changes the solvent parameters (polarity, composition) to control the desorption process. By using a gradient of solvents with increasing polarity (water, aqueous ethanol, aqueous methanol, acetonitrile), the method selectively desorbs different cannabinoid glycoside species based on their varying solubility characteristics, resolving the purification challenge.
2Ease of operation
If resins are used to separate cannabinoid species, then separation simplicity is improved, but selective desorption of specific glycoside species becomes challenging
Solution Approach 1:
The patent introduces dynamic control to the resin separation process by implementing sequential desorption with progressively changing solvent compositions. This dynamic approach allows selective elution of different cannabinoid glycoside species at different stages, transforming a static resin separation into a controlled, multi-stage purification process that achieves both simplicity and precision.
Solution Approach 2:
The patent systematically changes solvent parameters (composition, polarity, concentration) across multiple desorption steps to achieve selective separation. Each solvent condition is optimized to target specific glycoside species, enabling precise control over which compounds are desorbed at each stage while maintaining the simplicity of resin-based separation.
3Adaptability or versatility
If multiple cannabinoid glycoside species are present in complex mixture, then product diversity is improved, but purification cost and complexity increases
Solution Approach 1:
The patent divides the purification of complex mixtures into discrete, manageable segments corresponding to different glycoside classes (monoglycosides, diglycosides, triglycosides). Each segment is isolated through selective desorption, allowing independent purification and reducing the overall complexity compared to attempting to separate all components simultaneously.
Solution Approach 2:
The patent employs a universal resin-based platform that can handle diverse cannabinoid glycoside species through a standardized sequential desorption protocol. The same resin and methodology work for different glycoside types, reducing the need for multiple specialized purification systems and lowering overall complexity despite product diversity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method achieves high purity (95%-97%) and yield (60-90%) of CBD1G and CBD2G, reducing downstream processing costs and enhancing the commercial relevance of cannabinoid glycosides.
Implementation Method 1
strategies for product capture and purification of desired fermentation products remain underdeveloped in the public domain, especially at industrial scale... utilizing the solubility and lipophilic properties of CBD-glycoside species to effectively (i) desorb the products from absorbed resins
Implementation Method 2
utilizing the solubility and lipophilic properties of CBD-glycoside species to effectively (i) desorb the products from absorbed resins
Implementation Method 3
followed by liquid/liquid extraction and crystallization methods
Implementation Method 4
followed by liquid/liquid extraction and crystallization methods
Data Source
AI summary
High yield separation and purification of one or more cannabinoid glycoside species from a complex mixture of cannabinoids. In one preferred aspect, the novel systems and methods of the invention can separate one or more cannabinoids and/or cannabinoid glycoside species from a complex mixture, and preferably a quantity of cannabidiol (CBD) glycosides having two UDP-sugar moieties from a complex mixture of CBD and CBD-glycosides having one UDP-sugar moiety.