CCNY Inhibitor Memory Improvement via GluA1 Receptor Trafficking

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Solution Overview

Problem

The role of Cyclin Y (CCNY) in the mammalian nervous system and its regulation of synapse remodeling and memory formation was not well understood until recent research revealed its involvement in synapse elimination and formation, and its impact on memory-mediated receptors.

Innovation Solution

A composition containing a CCNY inhibitor, such as shRNA, siRNA, antisense nucleotides, or antibodies, is administered to inhibit CCNY expression or activity, thereby enhancing memory by regulating the surface expression of memory-mediating receptors like GluA1.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If CCNY function is decreased by shRNA expression, then surface delivery of GluA1 receptors is facilitated and memory is improved, but CCNY protein levels are reduced

Engineering Contradiction:

Improvememory functionVSAvoidCCNY protein level

Core Design Contradiction:

ReliabilityVSQuantity of substance

Solution Approach 1:

The invention extracts and removes CCNY protein from the system using shRNA-mediated gene silencing. The shRNA specifically targets and degrades CCNY mRNA, preventing CCNY protein synthesis. This extraction of the harmful CCNY protein allows GluA1 receptors to be properly delivered to the cell surface, thereby improving memory function without causing unwanted side effects of CCNY overexpression

Inventive Principle:

Principle #2Taking out (Extraction)

Solution Approach 2:

The invention changes the quantity parameter of CCNY protein by using varying concentrations and designs of shRNA. By adjusting the shRNA expression levels and targeting efficiency, the patent optimizes the reduction of CCNY protein to achieve the desired balance between improving GluA1 surface delivery and maintaining adequate cellular function

Inventive Principle:

Principle #35Parameter changes

2Productivity

If CCNY is overexpressed, then cell division regulation is enhanced, but surface delivery of GluA1 receptors is inhibited and memory problems occur

Engineering Contradiction:

Improvecell division regulationVSAvoidmemory function

Core Design Contradiction:

ProductivityVSReliability

Solution Approach 1:

Instead of trying to enhance GluA1 delivery while maintaining CCNY overexpression, the invention inverts the approach by reducing CCNY levels to achieve proper GluA1 surface delivery. The patent demonstrates that CCNY acts as a negative regulator of GluA1 trafficking, so inhibiting CCNY function through shRNA expression reverses the blocking effect and restores normal receptor surface delivery, thereby improving memory function

Inventive Principle:

Principle #13The other way round (Inversion)

Data Source

PatentUS10131909B2Method for improving memory using CCNY inhibitor

Publication Date: 2018.11.20 KOREA INST OF SCI & TECH

AI summary

The present invention relates to a composition for improving memory including a Cyclin Y (CCNY) inhibitor as an active ingredient. More specifically, the present invention relates to a method for improving memory in a subject comprising administering the composition for improving memory to the subject.The present invention may reveal a CCNY-oriented molecular mechanism with respect to learning and memory, help understand causes of brain diseases associated with memory problems, and ultimately be applied in the treatment and diagnosis of brain memory disorders such as dementia.