Skeletal Muscle Tissue Soaking for CD56 Cell Enrichment
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current methods for increasing the proportion of CD56 positive cells in skeletal muscle tissue for graft production are inefficient, as they require destructive enzyme treatments, leading to contamination with non-CD56 positive cells, which complicates the production of high-quality cell populations for treating severe heart failure.
Innovation Solution
Soaking skeletal muscle tissue in a preservation solution to reduce non-CD56 positive cells to 20% or less, achieving a degree of swelling of 1.2 or more, and maintaining the soaking for 12 hours or more to enhance the proportion of CD56 positive cells, allowing for their stable separation and use in graft production.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Manufacturing precision
If destructive enzyme treatment is used to separate CD56 positive cells from skeletal muscle tissue, then CD56 positive cells can be obtained, but the tissue is destroyed and contaminated with non-CD56 positive cells
Solution Approach 1:
The patent applies preliminary action by soaking skeletal muscle tissue in preservation solution before enzyme treatment to selectively reduce non-CD56 positive cells. This pre-treatment step creates a more favorable condition for subsequent cell separation, allowing CD56 positive cells to be enriched while minimizing contamination from non-CD56 positive cells.
Solution Approach 2:
The patent utilizes parameter changes by altering the preservation conditions (soaking time, preservation solution composition) to selectively affect non-CD56 positive cells. By controlling the soaking duration and solution properties, the method changes the physiological state of different cell types, enabling selective reduction of unwanted cells while preserving CD56 positive cells.
2Manufacturing precision
If skeletal muscle tissue is soaked in preservation solution for extended periods, then proportion of CD56 positive cells increases, but time and resource consumption increase
Solution Approach 1:
The patent optimizes the soaking parameters (time, temperature, preservation solution composition) to achieve the desired cell separation efficiency in a reasonable timeframe. By carefully adjusting these parameters, the method achieves effective enrichment of CD56 positive cells without requiring excessively long soaking periods, thus balancing precision with time efficiency.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method effectively increases the proportion of CD56 positive cells, providing a high-quality cell population for grafts that can be used to treat heart disease by maintaining the survival advantage of CD56 positive cells over non-CD56 positive cells during the soaking process.
Implementation Method 1
the proportion of CD56 positive cells in skeletal muscle tissue is dramatically increased when the sampled skeletal muscle tissue is soaked in a preservation solution
Data Source
AI summary
Methods for increasing a proportion of CD56 positive cells in sampled skeletal muscle tissue include soaking the sampled skeletal muscle tissue in a preservation solution.