CD9P-1 Peptide Fragments Targeting CD151 for Cancer Therapy

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Solution Overview

Problem

Current anti-angiogenic treatments for cancers and tumors, such as those targeting VEGF or CD9P-1, are inefficient and often cause harmful secondary effects, necessitating the development of alternative therapies with enhanced efficacy and reduced toxicity.

Innovation Solution

The use of specific peptides, P3 and P4, which are fragments of the CD9P-1 protein, that selectively target and degrade CD151, thereby inhibiting angiogenesis without affecting CD9 or CD9P-1 levels, and can be used alone or in combination with chemotherapeutic agents like cisplatin.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If current anti-angiogenic treatments (such as those targeting VEGF or CD9P-1) are used, then angiogenesis is inhibited, but harmful secondary effects occur and efficiency is insufficient

Engineering Contradiction:
Improvetreatment efficiencyVSAvoidsecondary effects
Core Design Contradiction:
ReliabilityVSObject-affected harmful factors

Solution Approach 1:

The patent segments the CD9P-1 protein into specific functional fragments (168A-T2 and its derivatives) that retain anti-angiogenic activity while removing portions responsible for harmful effects. This segmentation allows selective targeting of angiogenesis pathways without the systemic side effects of full-length protein or non-specific inhibitors.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The invention applies local quality by designing peptides with specific sequences (such as P3: GNYYCSVTPWVKS and P4: IHSKPVFITVKMDVLNA) that target particular molecular interactions in the angiogenesis pathway. These localized sequence modifications enable precise biological activity while minimizing off-target effects.

Inventive Principle:
Principle #3Local quality

2Reliability

If polypeptide fragments (168A-T2) are used to target CD9P-1, then anti-angiogenic activity is enhanced, but specificity must be maintained to avoid affecting other tetraspanins

Engineering Contradiction:
Improveanti-angiogenic activityVSAvoidtarget specificity
Core Design Contradiction:
ReliabilityVSManufacturing precision

Solution Approach 1:

The patent divides the 168A-T2 polypeptide into smaller, defined peptide fragments with specific amino acid sequences. This segmentation creates molecules small enough to achieve high specificity for CD9P-1 while large enough to maintain stable structural conformation required for biological activity.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The invention modifies physical-chemical parameters of the peptide fragments, including molecular weight, amino acid composition, and structural conformation, to optimize both specificity for CD9P-1 and potency of anti-angiogenic activity. Specific sequence parameters (such as disulfide bridge positions) are carefully controlled.

Inventive Principle:
Principle #35Parameter changes

Data Source

PatentEP3134428B1Polypeptide fragments of 168a-t2 and compositions comprising them for use in treating cancer
Publication Date: 2019.06.12 GENE SIGNAL INT SA
  • EP3134428B1 patent drawingFigure 1~2
  • EP3134428B1 patent drawingFigure 3~4
  • EP3134428B1 patent drawingFigure 5~6

AI summary

The present invention relates to specific polypeptides having anti-angiogenic activity and their use for treating cancer.