3',5'-cyclic diguanylic acid free acid crystallization
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Solution Overview
Problem
Conventional methods for obtaining 3',5'-cyclic diguanylic acid crystals are unsuitable for large-scale production in a short time and raise safety concerns due to the inclusion of metal salts, while methods for free acid crystals are unknown, limiting their pharmaceutical applications.
Innovation Solution
A method involving the addition of acid to an aqueous solution of 3',5'-cyclic diguanylic acid to lower the pH to 1 to 3, allowing for the crystallization of free acid crystals without metal salts, which are stable and easy to handle.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Manufacturing precision
If the vapor diffusion method is used to obtain 3’,5’-cyclic diguanylic acid crystals, then the crystals can be obtained with good quality, but the production efficiency is low and large amount of crystals cannot be obtained in a short period of time
Solution Approach 1:
The invention changes the crystallization parameters from vapor diffusion to acidification method. By lowering the pH to 1-3 through acid addition, the invention achieves rapid crystallization while maintaining crystal quality, thereby resolving the contradiction between manufacturing precision and productivity
Solution Approach 2:
The invention utilizes phase transition of 3’,5’-cyclic diguanylic acid from dissolved state to crystalline state through pH-induced precipitation. This phase transition approach enables rapid crystal formation compared to the slow vapor diffusion process, improving productivity while maintaining crystal quality
2Ease of manufacture
If co-crystals with metal salts (cobalt or magnesium) are used, then the crystals can be obtained easily, but safety problems arise when intended for pharmaceutical raw material applications
Solution Approach 1:
The invention extracts and eliminates the harmful metal salt components from the crystal structure. By using acidification to precipitate free acid crystals instead of co-crystals, the invention removes cobalt and magnesium salts while maintaining crystal formation, thereby resolving the safety concerns for pharmaceutical applications
Solution Approach 2:
The invention replaces the use of expensive and potentially harmful metal salts with simple acid additives. This substitution uses inexpensive acids (hydrochloric acid, sulfuric acid, or nitric acid) to achieve crystal precipitation without introducing harmful metal ions, resolving the safety issue while maintaining ease of manufacture
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method enables the production of stable, metal-ion-free 3',5'-cyclic diguanylic acid crystals with high purity and stability, suitable for pharmaceutical applications, and characterized by specific diffraction angles and thermal properties.
Implementation Method 1
adding acid to an aqueous solution of 3’,5’-cyclic diguanylic acid so as to lower pH to 1 to 3
Implementation Method 2
preparation is possible by adding acid to an aqueous solution of 3’,5’-cyclic diguanylic acid so as to lower pH to 1 to 3
Data Source
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AI summary
A crystal of free acid of 3',5'-cyclic diguanylic acid containing no metal salt with cobalt, magnesium or the like is provided. A method is sought for obtaining said crystal in a large amount and with ease. By a manufacturing method comprising a step of adding acid to an aqueous solution of 3',5'-cyclic diguanylic acid so as to lower pH to 1 to 3, crystals of 3',5'-cyclic diguanylic acid can be obtained in a large amount with ease. Said crystals are free acid crystals which do not contain a metal salt with cobalt, magnesium or the like.