CDPK-SuSy3 Regulation for Higher-Sugar Tomatoes Without Weight Loss
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Modern tomato varieties face a challenge in achieving higher sugar content without sacrificing fruit weight, as larger fruits often have lower soluble solids content due to the negative correlation between fruit size and sugar accumulation.
Innovation Solution
Regulation of CDPK8 and CDPK9 protein function and expression levels, along with SuSy3, through gene editing and promoter manipulation to enhance sugar synthesis pathways in tomato fruits, thereby increasing glucose, fructose, and citric acid content while maintaining fruit weight.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Weight of moving object
If selection of larger fruits is performed to increase fruit weight, then fruit weight is improved, but soluble solids content deteriorates
Solution Approach 1:
The patent changes the physiological state parameters of the fruit by regulating CDPK protein phosphorylation activity and SuSy3 gene expression levels. By controlling the phosphorylation state of SuSy3 protein through CDPK regulation, the patent optimizes sugar synthesis pathway efficiency, thereby increasing soluble solids content (SSC) without compromising fruit weight, directly resolving the trade-off between these two parameters
Solution Approach 2:
The patent introduces CDPK (calcium-dependent protein kinase) and SuSy3 (sucrose synthase) as intermediary regulatory elements between fruit development and sugar accumulation. By manipulating the phosphorylation activity of CDPK and the expression of SuSy3, the patent mediates the relationship between fruit growth and sugar content, enabling independent optimization of both fruit weight and soluble solids content
2Volume of moving object
If modern breeding for larger fruits is pursued, then fruit size is improved, but sugar content deteriorates
Solution Approach 1:
The patent changes the metabolic parameters of sugar synthesis by regulating CDPK phosphorylation activity and SuSy3 expression. This allows increased sugar content in large-fruited varieties through biochemical pathway optimization rather than traditional selection, breaking the negative correlation between fruit size and sugar content that plagues modern breeding
Solution Approach 2:
The patent replaces traditional mechanical breeding methods (selection and hybridization) with molecular-level regulation of CDPK and SuSy3. By using gene expression manipulation and protein phosphorylation control, the patent substitutes conventional breeding mechanisms with precise biochemical control, enabling sugar content enhancement in large fruits without relying on genetic recombination
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method results in sweeter tomatoes with increased soluble solids content and improved flavor without significant changes in fruit size, achieved by controlling the phosphorylation of SuSy3 protein and optimizing gene expression.
Implementation Method 1
The present disclosure discovered a calcium-dependent protein kinase 8 (SICDPK8), which is mainly expressed in mature fruits with fixed fruit size. The present disclosure found that, CDPK8 protein can interact with SuSy3 protein to regulate the stability of SuSy3 protein by phosphorylating it, thereby affecting the sugar content of tomato fruit.
Data Source
AI summary
Disclosed are a polynucleotide, a protein, a biological material, and use thereof in the improvement of the quality of a plant fruit. The polynucleotide includes any one of the sequences set forth in SEQ ID NOs: 3, 11, 23-36, and 95-108; a complementary sequence, a degenerate sequence, or a homologous sequence thereof; a sequence hybridized with the sequence or a complementary sequence thereof under rigorous conditions; and a cDNA sequence of the sequence. It is found that the gene is a key regulatory gene affecting the sugar content of the fruit, and in the use, the content of SSC, glucose, fructose, citric acid, and malic acid of the plant fruit can be regulated by regulating the protein function, the expression level, and the like of CDPK gene without significantly affecting the weight of the fruit, such that the quality and flavor of a tomato fruit are improved.