CE-7 Perhydrolase Formulation Buffering for Enzyme Stability
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Solution Overview
Problem
The stability of CE-7 enzymes with perhydrolysis activity is compromised when stored in organic solvents with a log P value less than two, leading to unacceptable loss of enzyme activity, as these solvents are detrimental to enzyme stability.
Innovation Solution
Incorporating a buffer into a formulation with a CE-7 enzyme and a carboxylic acid ester, and spray-drying the enzyme with an oligosaccharide excipient and optionally a surfactant to enhance the stability of the perhydrolysis activity, thereby stabilizing the enzyme even in the presence of organic ester substrates.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of manufacture
If CE-7 enzymes are stored in organic solvents with log P less than two, then the formulation can be created, but enzyme stability is compromised leading to unacceptable loss of activity
Solution Approach 1:
The patent introduces a mediator substance that is compatible with both the enzyme and the low log P organic solvent. This mediator acts as an intermediary that protects the enzyme from the detrimental effects of the solvent while allowing the formulation to be created and stored, thus resolving the contradiction between ease of manufacture and enzyme stability.
Solution Approach 2:
The patent modifies the formulation parameters by adjusting the composition to include protective agents or modifying the solvent system to reduce its harmful effects on the enzyme. This allows the formulation to maintain enzyme activity while still being manufacturable with low log P organic solvents.
2Stability of the object's composition
If multiple reaction components are combined to generate peracid prior to use, then peracid stability is improved, but the complexity of the formulation increases
Solution Approach 1:
The patent divides the peracid generation system into separate stable components that can be stored individually and combined before use. This segmentation allows each component to remain stable during storage while enabling peracid generation when needed, resolving the contradiction between stability and complexity by organizing the system into manageable segments.
Solution Approach 2:
The patent prepares the reaction components in advance in a stable form, separating the peracid generation step from the storage phase. By performing preliminary preparation of stable precursors and only combining them when peracid is needed, the system achieves both stability during storage and functional complexity only when required.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The addition of a buffer to the formulation significantly enhances the storage stability of CE-7 enzymes, maintaining a substantial portion of their perhydrolysis activity over extended periods, even in organic ester substrates, ensuring effective peracid production for disinfection and sanitization applications.
Implementation Method 1
the addition of at least one buffer to a formulation comprising a carboxylic acid ester and an enzyme powder comprising the CE-7 enzyme and at least one excipient enhances the stability of the perhydrolysis activity of the CE-7 enzyme stored in the formulation
Implementation Method 2
spray-drying the aqueous formulation of (a) to produce an enzyme powder comprising said at least one enzyme, said at least one oligosaccharide excipient, and optionally said at least one surfactant
Implementation Method 3
an enzyme powder comprising a spray-dried formulation of at least one enzyme structurally classified as a CE-7 enzyme and having perhydrolysis activity, at least one excipient, and optionally at least one surfactant
Data Source
AI summary
Disclosed herein is a method for stabilization of the perhydrolase activity of the CE-7 esterase in a formulation with a carboxylic acid ester that employs the addition of a buffering agent, substantially undissolved, to the mixture of the CE-7 esterase and the carboxylic acid ester. Further, disinfectant and laundry care formulations comprising the peracids produced by the processes described herein are provided.