CE-7 Perhydrolase Formulation Buffering for Ester Storage Stability
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Solution Overview
Problem
The stability of CE-7 enzymes with perhydrolysis activity is compromised when stored in organic solvents with a log P value less than two, leading to unacceptable loss of enzyme activity, which is detrimental for peracid production in disinfectant and bleaching applications.
Innovation Solution
Incorporating a buffer, such as sodium or potassium salts, into a formulation with a CE-7 enzyme and a carboxylic acid ester, and optionally a surfactant, to enhance the stability of the enzyme's perhydrolysis activity, and then spray-drying the mixture to produce an enzyme powder that retains activity when stored with the ester substrate.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of manufacture
If CE-7 enzymes are stored in organic solvents with log P less than two, then the formulation can be prepared with the enzyme and ester substrate, but the enzyme activity is lost over time
Solution Approach 1:
The patent introduces a buffer system (intermediary substance) between the enzyme and the organic solvent to mediate their interaction. The buffer maintains a stable pH environment that protects the enzyme from denaturation caused by low log P solvents, thereby preserving enzyme activity while allowing the formulation to be prepared with the ester substrate present.
2Device complexity
If the enzyme is stored in organic solvents for long term, then the formulation remains simple, but the enzyme activity decreases unacceptably
Solution Approach 1:
The patent changes the pH parameter of the formulation by adding a buffer system. This parameter change creates a protective environment for the enzyme that maintains its activity over extended storage periods in organic solvents, thereby extending the duration of enzyme action without significantly increasing formulation complexity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The addition of a buffer to the enzyme formulation significantly stabilizes the perhydrolysis activity of CE-7 enzymes, maintaining enzymatic activity for extended periods, even in organic solvents with low log P values, thereby ensuring consistent peracid production for disinfection and bleaching processes.
Implementation Method 1
The addition of at least one buffer to a formulation comprising a carboxylic acid ester and an enzyme powder comprising the CE-7 enzyme and at least one excipient enhances the stability of the perhydrolysis activity of the CE-7 enzyme stored in the formulation
Implementation Method 2
spray-drying the aqueous formulation of (a) to produce an enzyme powder comprising said at least one enzyme, said at least one excipient, and optionally said at least one surfactant
Implementation Method 3
The CE-7 family of carbohydrate esterases has recently been reported to have perhydrolase activity. These 'perhydrolase' enzymes have been demonstrated to be particularly effective for producing peracids from a variety of carboxylic acid ester substrates when combined with a source of peroxygen
Data Source
AI summary
Disclosed herein is a method for stabilization of the perhydrolase activity of the CE-7 esterase in a formulation with a carboxylic acid ester that employs the addition of a buffering agent, substantially undissolved, to the mixture of the CE-7 esterase and the carboxylic acid ester. Further, disinfectant and laundry care formulations comprising the peracids produced by the processes described herein are provided.