Spray-Dried CE-7 Enzyme Powder for Stable Peracid Generation
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Solution Overview
Problem
Peracid-based disinfectants and bleaching agents are unstable in solution over time, and enzymes used for peracid generation, such as those from the CE-7 family, lose activity when stored in organic solvents with low log P values, which are detrimental to enzyme stability.
Innovation Solution
A process involving spray-drying an aqueous formulation of CE-7 enzymes with oligosaccharide excipients to produce an enzyme powder that retains perhydrolase activity when combined with carboxylic acid esters, stabilizing the enzyme activity even in organic solvents with low log P values.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If peracid-based disinfectants are prepared as equilibrium mixtures of peracid, hydrogen peroxide, and carboxylic acid, then disinfection and bleaching efficacy is achieved, but the peracid becomes unstable in solution over time
Solution Approach 1:
The system is divided into separate stable components (carboxylic acid and hydrogen peroxide) that are stored independently and only combined at the point of use, preventing premature peracid formation and maintaining solution stability while ensuring disinfection efficacy when needed
Solution Approach 2:
The stable precursor components (carboxylic acid and hydrogen peroxide) are prepared and stored in advance in separate containers, ready for rapid mixing to generate the active peracid disinfectant when required, ensuring both stability during storage and efficacy during use
2Stability of the object's composition
If CE-7 enzymes are stored in organic solvents with low log P values to maintain formulation stability, then the organic phase stability is improved, but the enzyme loses perhydrolase activity
Solution Approach 1:
A phase transfer catalyst or surfactant intermediary is introduced to mediate between the organic solvent and the aqueous enzyme, enabling the enzyme to function in the organic phase without direct detrimental contact, thus maintaining both formulation stability and enzyme activity
Solution Approach 2:
The log P value of the organic solvent is optimized to a specific range that provides sufficient hydrophobicity for formulation stability while maintaining enough hydrophilicity or water content to preserve enzyme conformation and perhydrolase activity
3Stability of the object's composition
If multiple reaction components are kept separated until peracid solution is needed, then peracid instability issues are overcome, but the complexity of the formulation system increases
Solution Approach 1:
Multiple separate stable components (carboxylic acid, hydrogen peroxide, and enzyme) are combined into a single integrated formulation system that maintains stability through proper component selection and ratios, simplifying storage and handling while enabling effective peracid generation when activated
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The enzyme powder maintains significant perhydrolase activity for extended periods, enabling effective peracid production and use in disinfection, sanitization, and bleaching applications without stability issues.
Implementation Method 1
spray-drying an aqueous formulation of CE-7 enzymes with oligosaccharide excipients to produce an enzyme powder
Implementation Method 2
CE-7 enzymes with perhydrolase activity... producing peracids from a variety of carboxylic acid ester substrates
Data Source
AI summary
Disclosed herein are enzyme powders comprising a spray-dried formulation of at least one CE-7 esterase, at least one oligosaccharide excipient, and optionally at least one surfactant. Also disclosed herein is a process for producing peroxycarboxylic acids from carboxylic acid esters using the aforementioned enzyme powders. Further, disinfectant and laundry care formulations comprising the peracids produced by the processes described herein are provided.