Spray-Dried CE-7 Enzyme Powder for Low-LogP Solvent Stability
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Solution Overview
Problem
The stability of CE-7 enzymes with perhydrolysis activity is compromised when stored in organic solvents with a log P value less than two, leading to unacceptable loss of enzyme activity, which is detrimental for long-term storage in peracid generation systems.
Innovation Solution
A process involving the spray-drying of an aqueous formulation containing a CE-7 enzyme and an oligosaccharide excipient is used to produce an enzyme powder that retains perhydrolysis activity when combined with a carboxylic acid ester substrate, enhancing stability and activity retention.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If CE-7 enzyme is stored in organic solvents with log P less than two, then the enzyme can be used for peracid generation, but the enzyme activity is lost over time
Solution Approach 1:
The patent introduces a surfactant as an intermediary substance between the CE-7 enzyme and the organic solvent. The surfactant forms a protective interface that prevents direct harmful interaction between the low log P solvent and the enzyme, thereby maintaining enzyme activity during storage while still allowing the system to function for peracid generation.
Solution Approach 2:
The patent creates a composite formulation consisting of the CE-7 enzyme, organic solvent with low log P value, and surfactant. This composite system allows the enzyme to remain stable in the organic phase by forming a protective complex or micellar structure, enabling both long-term storage stability and functional activity.
2Reliability
If multiple reaction components are combined to generate peracid prior to use, then peracid stability is improved, but the complexity of the formulation increases
Solution Approach 1:
The patent combines multiple reaction components (CE-7 enzyme, organic solvent, surfactant, and peracid precursor) into a single stable formulation that can be stored as one product. The components remain compatible and stable together, allowing the system to generate peracid in situ when needed without requiring separate storage of multiple components.
Solution Approach 2:
The patent performs preliminary stabilization of the enzyme and reaction components by formulating them together with appropriate excipients and surfactants before use. This preliminary formulation ensures that when the product is applied, the peracid generation occurs immediately and efficiently without requiring additional preparation steps.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The enzyme powder maintains significant perhydrolysis activity over extended storage periods, even in the presence of organic solvents with low log P values, ensuring stable peracid production and effective disinfection or sanitization applications.
Implementation Method 1
spray-drying of an aqueous formulation containing a CE-7 enzyme and an oligosaccharide excipient is used to produce an enzyme powder
Implementation Method 2
CE-7 enzyme having perhydrolysis activity
Implementation Method 3
The enzymatic peracid generation system... CE-7 carbohydrate esterases have been demonstrated to have perhydrolytic activity sufficient to produce peracetic acid from acetyl esters
Data Source
AI summary
Disclosed herein are enzyme powders comprising a spray-dried formulation of at least one CE-7 esterase, at least one oligosaccharide excipient, and optionally at least one surfactant. Also disclosed herein is a process for producing peroxycarboxylic acids from carboxylic acid esters using the aforementioned enzyme powders. Further, disinfectant and laundry care formulations comprising the peracids produced by the processes described herein are provided.