Buffered CE-7 Esterase Formulation for Perhydrolase Storage Stability
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Solution Overview
Problem
The stability of CE-7 enzymes with perhydrolysis activity is compromised when stored in organic solvents with a log P value less than two, leading to unacceptable loss of enzyme activity, which is detrimental for long-term storage in peracid generation systems.
Innovation Solution
Incorporating a buffer into a formulation comprising a CE-7 enzyme and a carboxylic acid ester, along with an excipient and optionally a surfactant, and spray-drying the enzyme powder to enhance the stability of the perhydrolysis activity, thereby stabilizing the enzyme even in organic ester substrates.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If CE-7 enzymes are stored in organic solvents with log P < 2, then the formulation can be compact and易于 storage, but the enzyme activity is significantly compromised
Solution Approach 1:
The patent introduces a mediator substance (such as a surfactant or cosolvent) that bridges the incompatible environment of organic solvents and the enzyme's aqueous nature. This intermediary protects the enzyme from direct contact with harmful organic solvents while allowing the formulation to remain compact and storable.
Solution Approach 2:
The patent modifies the physical-chemical parameters of the storage environment by adjusting solvent composition, adding stabilizing agents, or controlling storage conditions (temperature, pH) to maintain enzyme activity while preserving formulation compactness for long-term storage.
2Stability of the object's composition
If multiple reaction components are combined to generate peracid prior to use, then peracid stability is improved, but the complexity of the formulation increases
Solution Approach 1:
The patent divides the peracid generation system into separate functional components (enzyme component, substrate component, activator component) that can be stored independently and combined only when needed. This segmentation maintains peracid stability during storage while simplifying individual component formulations.
Solution Approach 2:
The patent prepares reaction components in advance in stable, separate forms (lyophilized powders, stable precursors) that can be quickly reconstituted or activated when peracid generation is needed, avoiding the complexity of stabilizing pre-mixed peracid solutions.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The addition of a buffer to the formulation significantly enhances the stability of the perhydrolysis activity of CE-7 enzymes, maintaining enzymatic activity for extended periods, even in organic ester substrates, thus overcoming the stability issues associated with storage.
Implementation Method 1
the addition of at least one buffer to a formulation comprising a carboxylic acid ester and an enzyme powder comprising the CE-7 enzyme and at least one excipient enhances the stability of the perhydrolysis activity of the CE-7 enzyme stored in the formulation
Implementation Method 2
spray-drying the enzyme powder to enhance the stability of the perhydrolysis activity
Data Source
AI summary
Disclosed herein is a method for stabilization of the perhydrolase activity of the CE-7 esterase in a formulation with a carboxylic acid ester that employs the addition of a buffering agent, substantially undissolved, to the mixture of the CE-7 esterase and the carboxylic acid ester. Further, disinfectant and laundry care formulations comprising the peracids produced by the processes described herein are provided.