CE-7 Peracid Formulation for Solubility and Enzyme Stability
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Solution Overview
Problem
Multi-component enzymatic peroxycarboxylic acid generation systems face issues with the solubility of carboxylic acid ester substrates, leading to high viscosity and poor mixing efficiency, which affects the production and delivery of peroxycarboxylic acid, and the stability of CE-7 enzymes is compromised by organic cosolvents with low log P values.
Innovation Solution
A multi-component formulation comprising a CE-7 enzyme catalyst with specific motifs and a carboxylic acid ester substrate, along with a cosolvent and buffer, is designed to enhance storage stability and mixing characteristics, using oligosaccharides and surfactants to improve solubility and enzyme activity, and a two-component system with hydrogen peroxide and a stabilizer is used to produce peroxycarboxylic acid.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If carboxylic acid ester substrates are used in enzymatic peroxycarboxylic acid generation systems, then peroxycarboxylic acid production is enabled, but high viscosity and poor mixing efficiency occur due to solubility issues
Solution Approach 1:
The patent introduces cosolvents as intermediary substances to mediate between the carboxylic acid ester substrates and the aqueous enzyme solution. These cosolvents have affinity for both organic esters and water, enabling improved solubility and mixing without compromising enzyme activity or peroxycarboxylic acid production efficiency
Solution Approach 2:
The patent modifies the physical and chemical parameters of the reaction system by adjusting cosolvent composition, substrate concentration, and pH conditions. These parameter changes optimize the balance between substrate solubility, mixture viscosity, and enzymatic reaction efficiency, resolving the contradiction between productivity and ease of operation
2Ease of operation
If organic cosolvents with low log P values are used to improve substrate solubility, then mixing efficiency is improved, but CE-7 enzyme stability is compromised
Solution Approach 1:
The patent carefully selects and adjusts the log P values of cosolvents to fall within an optimal range that provides sufficient substrate solubility while maintaining enzyme stability. By precisely controlling this parameter, the patent resolves the contradiction between mixing efficiency and enzyme reliability
Solution Approach 2:
The patent uses cosolvents as intermediary substances that bridge the hydrophobic substrate and hydrophilic enzyme environment. These intermediaries improve mixing efficiency without directly contacting and denaturing the enzyme, thus preserving enzyme stability while achieving good mixing
3Productivity
If high concentration of carboxylic acid ester substrate is used, then peroxycarboxylic acid production increases, but viscosity increases and mixing becomes difficult
Solution Approach 1:
The patent introduces cosolvents as intermediary substances that reduce mixture viscosity at high substrate concentrations. These cosolvents solvate the ester substrates and disrupt intermolecular associations, maintaining low viscosity even at high substrate levels, thereby enabling both high productivity and easy mixing
Solution Approach 2:
The patent adjusts the composition parameters including cosolvent ratio, substrate concentration, and temperature to optimize the balance between productivity and viscosity. By controlling these parameters, the patent achieves high peroxycarboxylic acid production while maintaining manageable mixture viscosity for efficient mixing and delivery
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The formulation achieves enhanced storage stability and improved mixing and viscosity characteristics, enabling efficient production and delivery of peroxycarboxylic acid, while maintaining enzyme activity and stability, suitable for disinfection, bleaching, and sanitization applications.
Implementation Method 1
an enzyme catalyst having perhydrolysis activity... converting carboxylic acid esters (in the presence of a suitable source of peroxygen, such as hydrogen peroxide) into peroxycarboxylic acids
Implementation Method 2
Peroxycarboxylic acids can be prepared by the chemical reaction of a carboxylic acid and hydrogen peroxide... The reaction is usually catalyzed by a strong inorganic acid
Implementation Method 3
along with a cosolvent and buffer, is designed to enhance storage stability and mixing characteristics, using oligosaccharides and surfactants to improve solubility
Data Source
AI summary
Disclosed herein are multi-component formulations for enzymatically producing aqueous solutions of peroxycarboxylic acids suitable for use in, e.g., disinfectant and/or bleaching applications. The multi-component peroxycarboxylic acid formulations comprise at least one carbohydrate esterase family 7 enzyme having perhydrolytic activity.