Cell Accommodating Chip Polymer Coating for Precise Cell Isolation
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Solution Overview
Problem
Current cell accommodating chips face challenges in accurately and efficiently identifying and isolating specific cells from a large number of cells without damaging them, due to issues with cell adhesion and the stability of binding materials, leading to reduced accuracy and efficiency in cell collection.
Innovation Solution
A cell accommodating chip with a substrate coated by a polymer having a crosslinked structure, including specific structural units derived from monomers, which provides low cell adhesion properties and affinity to specific binding materials, allowing for precise identification and collection of target cells without pre-binding of antibodies and minimizing damage.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If anti-Ig antibody is bound to chip upper surface for target sample identification, then detection capability is improved, but chip must be kept wet continuously and anti-Ig antibody cannot be sustained for long time
Solution Approach 1:
The chip surface is pre-coated with a polymer layer having low cell adhesion properties and low nonspecific binding characteristics before the experiment. This preliminary surface preparation prevents cell adhesion and maintains a stable environment for antibody binding and target detection without requiring continuous wetting maintenance.
2Measurement precision
If cell is accommodated in well for identification, then target sample detection is enabled, but cell may adhere to inner surface of well during antibody secretion making collection difficult
Solution Approach 1:
The chip surface properties are modified by coating with a polymer layer having specific characteristics: low cell adhesion properties and low nonspecific binding. This parameter change in surface chemistry prevents cells from adhering to the well inner surfaces while maintaining their viability and detectability, enabling easy collection without damage.
3Reliability
If functional coating is prepared on plate-like support for selective binding, then target sample binding is achieved, but structure cannot retain single cell in each well reducing accuracy and efficiency
Solution Approach 1:
The chip is divided into multiple discrete wells, each capable of accommodating and retaining a single cell. This segmentation allows independent control and observation of each cell, enabling high-accuracy identification and isolation. The polymer-coated surface within each well maintains low cell adhesion properties, preventing cells from adhering to the well walls and ensuring they remain contained and collectable.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The chip enables high-accuracy and high-efficiency identification and isolation of target cells by preventing cell adhesion and maintaining a wet environment, reducing nonspecific adsorption, and allowing for efficient cell collection without the need for blocking reagents.
Implementation Method 1
a surface of the cell accommodating chip having the plurality of wells is coated with a polymer having a crosslinked structure and including a structural unit derived from a monomer represented by General Formula (1), a structural unit derived from a monomer represented by General Formula (2), and a structural unit derived from a monomer represented by General Formula (3)
Implementation Method 2
the surface of the cell accommodating chip having the plurality of wells is coated with a polymer having a crosslinked structure and including a structural unit derived from a monomer represented by General Formula (1)... having low cell adhesion properties and having affinity to a specific binding material
Implementation Method 3
a surface of the cell accommodating chip having the plurality of wells is coated with a polymer having a crosslinked structure
Data Source
AI summary
A cell accommodating chip is adapted for use in a screening apparatus for searching for a predetermined cell based on optical information emitted from a substance on the cell accommodating chip and selectively collecting the cell searched for. The cell accommodating chip includes a substrate composed of a light-transmitting material, and a plurality of wells capable of accommodating cells, the plurality of wells being provided on at least one of main faces of the substrate. A surface of the cell accommodating chip having the plurality of wells is coated with a specific polymer having a crosslinked structure.