Cell Analyzer Multi-Step Dispersion for Aggregated Cells
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Solution Overview
Problem
Conventional cell analyzers face accuracy issues when analyzing highly aggregated cells in biological specimens, leading to impaired analysis results.
Innovation Solution
A cell analyzer is designed with multiple dispersion processes, including a shearing force applying process and an ultrasonic dispersion process, to effectively disperse aggregated cells into single cells, ensuring accurate detection and analysis.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If a single dispersion process is used on highly aggregated cells, then the device complexity is low, but the cell dispersion effectiveness is insufficient leading to impaired analysis accuracy
Solution Approach 1:
The dispersion process is segmented into multiple distinct steps: a first dispersion process using mechanical agitation and a second dispersion process using ultrasonic waves. Each step targets different aspects of cell aggregation, with the first process breaking up large aggregates and the second process dispersing remaining clumps, thereby achieving thorough dispersion that improves analysis accuracy
Solution Approach 2:
A dispersion促进 agent is introduced as an intermediary substance to facilitate the dispersion process. This agent is added to the biological specimen before dispersion and helps reduce cell aggregation by interfering with cellular adhesion mechanisms, making the subsequent mechanical and ultrasonic dispersion steps more effective
2Measurement precision
If multiple dispersion processes are applied, then the cell dispersion effectiveness improves, but the processing time increases
Solution Approach 1:
The dispersion processes are applied in periodic sequence rather than continuously. The first dispersion process is applied for a predetermined time, then the second dispersion process is applied for another predetermined time. This periodic application allows each process to work optimally without excessive duration, balancing effectiveness with time efficiency
Solution Approach 2:
The dispersion促进 agent is added in advance before the dispersion processes begin, allowing it to start working on reducing cell aggregation beforehand. This preliminary action reduces the intensity and duration needed for the subsequent mechanical and ultrasonic dispersion steps, thereby reducing total processing time
3Measurement precision
If strong dispersion forces are used to disperse highly aggregated cells, then the dispersion effectiveness improves, but the cell damage increases
Solution Approach 1:
The dispersion force application is segmented into two stages with increasing intensity. The first dispersion process uses milder mechanical agitation to break up large aggregates without excessive force. The second dispersion process then applies stronger ultrasonic waves to disperses remaining smaller clumps. This segmented approach achieves thorough dispersion while limiting cumulative cell damage by not applying maximum force throughout the entire process
Solution Approach 2:
The dispersion促进 agent is applied preliminarily to reduce cell aggregation before the mechanical and ultrasonic dispersion processes. This preliminary action weakens the aggregation forces, so that subsequent dispersion steps require less intense forces to achieve the same level of cell separation, thereby reducing cell damage from the dispersion forces
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The multi-step dispersion process enhances the accuracy of cell analysis by thoroughly dispersing cells, even when they are highly aggregated, resulting in reliable detection and analysis of cancerous or atypical cells.
Implementation Method 1
the first dispersion process is a shearing force applying process of applying a shearing force to aggregated cells
Implementation Method 2
the second dispersion process is an ultrasonic dispersion process of dispersing aggregated cells by using ultrasonic waves
Data Source
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AI summary
Provided is a cell analyzer that can perform highly accurate cell analysis even when cells in a biological specimen are aggregated at a high level. A cell analyzer 1 includes: a cell dispersion unit which causes aggregated cells in a biological specimen to be dispersed, through a first dispersion process and a second dispersion process which is different from the first dispersion process; a primary detection unit 21 which detects characteristics information reflecting properties of the cells in the biological specimen on which the first dispersion process and the second dispersion process have been performed; and a data processing apparatus 4 which analyzes the cells in the biological specimen, based on a detection result from the primary detection unit 21.