Cell Analyzer Temperature Regulation for Optical Signal Stability
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Solution Overview
Problem
The variability in preservation temperatures of cell samples during transportation affects the accuracy of optical information obtained in cell analysis, leading to inconsistent results in cancer diagnosis.
Innovation Solution
Regulating the temperature of the medium containing cells to a range of 35°C to 45°C before analysis, using a temperature regulating unit in the cell analyzer, to stabilize optical signals and reduce variability.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If cells are preserved in alcohol-containing medium at variable temperatures during transportation, then the preservation and transport process is simple and flexible, but the optical information obtained during analysis shows variability and reduced accuracy
Solution Approach 1:
The patent applies parameter changes by regulating the temperature of the alcohol-containing medium to a specific range (35°C to 45°C) before analysis. This temperature regulation standardizes the physical state of the cells and medium, eliminating the variability caused by different preservation temperatures during transport. The parameter change from variable temperature to controlled temperature range directly resolves the contradiction between preservation flexibility and measurement accuracy.
2Adaptability or versatility
If the preservation temperature of the medium is varied according to different conditions, then the transport and storage process is adaptable to different environments, but the optical information obtained from cells shows inconsistency
Solution Approach 1:
The patent implements preliminary action by regulating the temperature of the medium to 35°C to 45°C before the analysis process begins. This pre-treatment step standardizes the sample conditions regardless of the varying preservation temperatures during transport and storage. By performing this temperature regulation action beforehand, the patent ensures that all samples start from a consistent thermal state, thereby guaranteeing reliable and consistent analysis results.
3Productivity
If no temperature regulation is applied to the medium, then the analysis process is simpler and faster, but the optical information obtained shows variability due to different preservation temperatures
Solution Approach 1:
The patent applies parameter changes by introducing temperature regulation (35°C to 45°C) as a controlled step in the analysis process. While this adds a processing step, the regulation time is optimized to be minimal, and the temperature range is set to accelerate the stabilization process. This parameter change resolves the contradiction by achieving both adequate measurement precision and maintained productivity, as the controlled temperature speeds up the stabilization of optical properties compared to uncontrolled variable temperatures.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach ensures consistent and accurate optical information, enhancing the reliability of cancer diagnosis by minimizing the impact of preservation temperature fluctuations on analysis results.
Implementation Method 1
regulating a temperature of a medium comprising alcohol, wherein the cell is fixed by the medium
Implementation Method 2
obtaining a fluorescent light information by irradiating light from a light source of the flow cytometer on the cell
Data Source
Figure 1
Figure 2
Figure 3A~3B
AI summary
The present invention provides a method for analyzing a cell. The method comprises steps of: regulating a temperature of a medium to a predetermined degree, wherein the cell is preserved in the medium; and obtaining an optical information by irradiating light on the cell.