Cell Analyzing Device Colony Size and Occupancy Rate
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Solution Overview
Problem
Existing cell analysis systems lack accuracy in determining the state of cultured cells, particularly in specifying the timing for differentiation induction of stem cells, as they rely solely on confluency or colony size without considering both factors simultaneously.
Innovation Solution
A cell analyzing device that acquires images of cultured cells, calculates the occupancy rate and size of colonies, and determines the state of cells based on both confluency and colony size, providing a comprehensive assessment for determining the optimal timing for differentiation induction.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If cell state determination relies solely on confluency or colony size, then the analysis method is simple, but the accuracy of cell state determination is insufficient
Solution Approach 1:
The patent combines multiple parameters (confluency, colony size, and their ratio) into a comprehensive cell state determination system. By merging these parameters and calculating their relationships, the system achieves more accurate cell state assessment while managing complexity through integrated analysis rather than separate simple measurements.
Solution Approach 2:
The patent introduces a new dimension of analysis by calculating the ratio between confluency and colony size, which provides additional information about cell state. This dimensional approach allows the system to distinguish between different cell states that would be indistinguishable using single-parameter analysis, thereby improving measurement precision.
2Measurement precision
If only single parameter (confluency or colony size) is used for analysis, then the calculation is simple, but the timing determination for differentiation induction is inaccurate
Solution Approach 1:
The system uses the calculated ratio of confluency to colony size as a feedback indicator to determine optimal differentiation timing. This feedback mechanism allows continuous monitoring and adjustment of the analysis, improving timing determination accuracy by providing a dynamic metric that reflects actual cell state changes over time.
Solution Approach 2:
The patent transforms the analysis from using fixed single parameters to using a derived ratio parameter that changes dynamically based on cell culture conditions. This parameter change enables more precise timing determination as the ratio reflects the relative rates of confluency increase and colony growth, providing a more sensitive indicator for differentiation timing.
Data Source
AI summary
A cell analyzing device includes a control circuit. The control circuit acquires data of an observation image. The control circuit specifies a colony of a cultured cell in the observation image. The control circuit calculates a ratio at which the cultured cell occupies a predetermined range included in the observation image as an occupancy rate. The control circuit calculates a size of the colony. The control circuit determines a state of the cultured cell based on the occupancy rate and the size of the colony.