Cell Analyzing Apparatus Using FFT Frequency Analysis

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Solution Overview

Problem

Current methods for diagnosing cancerous cells during operations are time-consuming and require skilled technicians, and existing cell analysis technologies do not provide rapid and accurate enough results for distinguishing between normal and cancerous tissues.

Innovation Solution

A cell analyzing apparatus and method that uses a histogram acquirer to measure fluorescence intensity and an analysis controller to perform Fast Fourier Transform (FFT) frequency analysis, determining the presence of cancer cells by comparing the area under the curve, ripple patterns, and steep attenuation in spatial frequency, allowing for objective and rapid diagnosis.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Loss of time

If conventional pathological analysis methods are used, then diagnostic accuracy can be maintained, but diagnosis time is excessively long and requires skilled technicians

Engineering Contradiction:

Improvediagnosis timeVSAvoiddiagnostic accuracy

Core Design Contradiction:

Loss of timeVSMeasurement precision

Solution Approach 1:

The patent replaces manual pathological analysis with an automated cell analyzing apparatus that uses flow cytometry and frequency analysis algorithms. The system automatically measures fluorescence intensity of nuclear stained cells and applies Fast Fourier Transform to histograms, eliminating the need for skilled technicians while maintaining diagnostic accuracy through objective computational analysis.

Inventive Principle:

Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The patent transforms the diagnostic approach by changing from visual histological examination to quantitative fluorescence intensity measurement. By measuring fluorescence intensity parameters and analyzing their frequency distribution through FFT, the system achieves rapid automated diagnosis while preserving accuracy through mathematical analysis of cellular parameters.

Inventive Principle:

Principle #35Parameter changes

2Loss of time

If rapid frozen section diagnosis is performed, then diagnosis time is reduced, but diagnostic accuracy and skill requirements increase

Engineering Contradiction:

Improvediagnosis timeVSAvoidskill requirement

Core Design Contradiction:

Loss of timeVSEase of operation

Solution Approach 1:

The patent replaces the skilled technician's visual assessment with an automated system that performs flow cytometry measurements and frequency analysis. The apparatus objectively analyzes fluorescence intensity histograms using Fast Fourier Transform algorithms, eliminating skill requirements while enabling rapid diagnosis without frozen sections.

Inventive Principle:

Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The system performs self-diagnosis by automatically measuring cell fluorescence intensity, generating histograms, applying frequency analysis, and determining malignancy grades without human intervention. The apparatus serves itself by integrating sample processing, measurement, analysis, and diagnosis into an autonomous workflow.

Inventive Principle:

Principle #25Self-service

3Productivity

If conventional histological examination is used, then comprehensive tissue analysis is possible, but the process is time-consuming and subjective

Engineering Contradiction:

Improvediagnosis speedVSAvoiddiagnosis objectivity

Core Design Contradiction:

ProductivityVSReliability

Solution Approach 1:

The patent replaces subjective visual histological examination with objective automated fluorescence measurement and frequency analysis. The system quantitatively measures nuclear stained cell fluorescence intensity and applies Fast Fourier Transform to histograms, providing reliable objective results at high speed without human subjectivity.

Inventive Principle:

Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The system incorporates feedback through automated measurement and analysis loops that continuously process cell fluorescence data. By measuring fluorescence intensity, generating histograms, applying frequency analysis, and comparing results against reference values, the system provides consistent objective feedback for rapid reliable diagnosis.

Inventive Principle:

Principle #23Feedback

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

Enables accurate and rapid distinction between cancerous and normal cells, reducing diagnosis time to about ten minutes and improving diagnostic accuracy by analyzing fluorescence intensity histograms and FFT patterns.

Implementation Method 1

a flow cytometer which measures a number of cells which are isolated and nuclear stained, and which obtains a histogram, of a fluorescence intensity

Methodology Applied

Scientific EffectFluorescence: Fluorescence

Data Source

PatentUS9448228B2Cell analyzing apparatus and cell analyzing method

Publication Date: 2016.09.20 NIHON KOHDEN CORP

AI summary

A cell analyzing apparatus includes: a histogram acquirer which is configured to perform measurement of a number of nuclear stained cells, and which, by using a result of the measurement, is configured to acquire a histogram indicating a fluorescence intensity; and an analysis controller which is configured to apply frequency analysis on data of the histogram acquired by the histogram acquirer, and which is configured to determine existence/nonexistence of cancer cells based on a result of the frequency analysis.