Cell Concentration Apparatus for Reducing Analysis Time
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Solution Overview
Problem
Conventional cell analyzers require a larger sample volume for accurate analysis, leading to increased analysis time and reagent consumption, necessitating a technique to concentrate cells in the sample for improved measurement efficiency.
Innovation Solution
A sample preparation apparatus with a storage chamber, a concentrated sample storage chamber, and an analysis target transportation section that separates and concentrates cells, allowing for a higher concentration of analysis targets, thereby reducing the sample volume needed for analysis.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If the number of cells to be analyzed is increased to improve measurement accuracy, then measurement accuracy is improved, but analysis time increases and reagent consumption increases
Solution Approach 1:
The patent changes the concentration parameter of the sample by separating and concentrating the analysis target cells. The cell concentration in the measurement sample is increased from the original low concentration to a higher concentration, allowing accurate analysis with fewer cells and thus reducing analysis time and reagent consumption while maintaining measurement accuracy.
2Measurement precision
If the number of cells to be analyzed is increased to improve measurement accuracy, then measurement accuracy is improved, but reagent consumption increases
Solution Approach 1:
The patent changes the concentration parameter of the sample by separating and concentrating the analysis target cells. The cell concentration in the measurement sample is increased from the original low concentration to a higher concentration, allowing accurate analysis with fewer cells and thus reducing analysis time and reagent consumption while maintaining measurement accuracy.
3Measurement precision
If a filter with small holes is used to separate cells, then cell separation is improved, but the filter clogs easily
Solution Approach 1:
The patent introduces a dynamic washing mechanism where the filter surface is periodically cleaned by flowing liquid through the filter in the opposite direction to the cell flow. This dynamic washing prevents filter clogging by removing accumulated cells and debris, allowing the filter to maintain its separation function over extended operation without requiring frequent replacement or causing measurement interruptions.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The apparatus enables faster analysis with reduced reagent consumption by concentrating cells, improving measurement accuracy and efficiency without increasing the sample volume, thus enhancing the analysis speed and reducing costs.
Implementation Method 1
the filter is provided at a lower end face of the cylindrical body, and the liquid is designed to be separated into a first liquid including the analysis target to be analyzed and a second liquid including a non_analysis target having a smaller diameter than that of the analysis target
Data Source
AI summary
A sample preparation apparatus comprising: a storage chamber that can store therein a liquid sample including an analysis target to be analyzed; a concentrated sample storage chamber that is provided to communicate with the storage chamber and that stores therein concentrated liquid having an analysis target having a higher concentration than that of the liquid sample; and an analysis target transportation section for transporting the analysis target included in the liquid sample stored in the storage chamber to the concentrated sample storage chamber. A cell analyzer is also disclosed.