Cell Concentration Control in Liquid Medium Processing
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Solution Overview
Problem
Existing devices for processing initial liquid media with cells are limited in their ability to adapt between in-vivo and in-vitro processing and fail to efficiently control flow rates to achieve desired product concentrations, particularly in varying in-vitro processing conditions.
Innovation Solution
The device incorporates a control unit that adjusts the flow rates of both the initial and washing solutions based on real-time cell concentration measurements, allowing for proportional control of the centrifuge's rotational speed and flow rates to achieve a product liquid medium with a consistent or maximized cell concentration, enabling both in-vivo and in-vitro processing.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If a fixed flow rate of solution is supplied via the second supply system, then the device can be operated simply, but the device cannot adapt to varying in-vitro processing conditions and achieve consistent product concentration
Solution Approach 1:
The patent implements dynamic flow rate control by adjusting the flow rate of solution via the second supply system based on real-time cell concentration measurements. The control unit modifies the flow rate parameter during operation, transitioning from a static fixed flow rate to a dynamic adaptive flow rate that responds to changing processing conditions, thereby resolving the contradiction between adaptability and complexity.
Solution Approach 2:
The patent employs a feedback control mechanism where a sensor measures the cell concentration in the initial liquid medium, and the control unit uses this measurement to adjust the flow rate of solution supplied via the second supply system. This closed-loop feedback system enables the device to adapt to varying in-vitro processing conditions while maintaining consistent product concentration, effectively resolving the technical contradiction.
2Manufacturing precision
If the flow rate of solution via the second supply system is reduced when cell concentration increases, then consistent product concentration is achieved, but the control system becomes more complex
Solution Approach 1:
The patent implements a feedback control system where the sensor continuously monitors cell concentration and the control unit adjusts the flow rate of solution via the second supply system accordingly. When cell concentration increases, the control unit reduces the flow rate to maintain consistent product concentration, resolving the contradiction between manufacturing precision and control system complexity through automated feedback regulation.
Solution Approach 2:
The patent changes the flow rate parameter of the solution supplied via the second supply system based on measured cell concentration. By dynamically adjusting this parameter in response to concentration changes, the system achieves consistent product concentration while the control unit manages the complexity of parameter adjustment, resolving the technical contradiction.
3Adaptability or versatility
If the device is designed for in-vivo processing only, then the design is simplified, but the device cannot perform in-vitro processing with varying conditions
Solution Approach 1:
The patent designs the device with multi-functionality to perform both in-vivo and in-vitro processing modes. By incorporating a controllable flow rate system via the second supply system that can be adjusted based on processing mode and cell concentration, the device achieves universality, enabling it to handle varying in-vitro conditions while maintaining the capability for standard in-vivo processing, thus resolving the contradiction between adaptability and design complexity.
Solution Approach 2:
The patent implements dynamic control capabilities that allow the device to adapt its operation based on the processing mode (in-vivo or in-vitro). The control unit adjusts parameters such as flow rate of solution via the second supply system according to the selected mode and measured cell concentration, enabling the device to function effectively in both modes without requiring separate dedicated systems, thereby resolving the contradiction between versatility and complexity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This solution enables the device to efficiently process initial liquid media, achieving a product liquid medium with consistent or maximized cell concentration, improving the adaptability and efficiency of cell separation across different processing conditions.
Implementation Method 1
measuring a physical parameter related to the concentration of the cells in the initial liquid medium using a sensor
Implementation Method 2
separating the initial liquid medium into various constituents including the product liquid medium
Implementation Method 3
separating the initial liquid medium into various constituents including the product liquid medium
Data Source
AI summary
A method for processing an initial liquid medium including cells in an initial concentration to obtain a product liquid medium including cells in a product concentration includes supplying the initial medium to a separator via a first supply system, separating the initial medium into various constituents including the product medium, extracting the product medium from the separator via a first outlet system, measuring a physical parameter related to the concentration of the cells in the initial medium using a sensor, and controlling at least one process parameter as a function of the physical parameter. During the step of separating the initial medium, a solution is supplied to the separator at a flow rate via a second supply system. The flow rate of the solution is determined based on the at least one process parameter, and is reduced when the concentration of the cells measured in the initial medium increases.