Cell Culture Atmosphere Control for Immune Cell Phenotype Modulation
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Solution Overview
Problem
Conventional cell culture incubators fail to replicate the native physiological conditions of cells, leading to altered gene and protein expression and diminished effectiveness of immune cells like T cells when reintroduced to patients, necessitating improved methods for culturing cells under hypoxic and hyperbaric conditions to enhance their therapeutic efficacy.
Innovation Solution
Culturing cells under specific oxygen and pressure conditions, ranging from 1% to 15% oxygen and up to 2 PSI above atmospheric pressure, to alter cytokine expression and enhance cytotoxicity in PBMCs, Tregs, and NK cells, thereby optimizing their therapeutic potential.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional cell culture incubators are used with ambient atmospheric conditions (20% oxygen, atmospheric pressure), then the incubation process is simple and equipment requirements are standard, but the cells fail to replicate native physiological conditions leading to altered gene and protein expression and diminished therapeutic efficacy
Solution Approach 1:
The patent applies parameter changes by modifying the atmospheric conditions within the incubator - specifically controlling oxygen levels (1-15% range) and pressure (up to 2 PSI above atmospheric) to replicate in vivo physiological conditions. This resolves the contradiction by changing the physical parameters of the culture environment to improve cell therapeutic efficacy while using a standard incubator platform.
2Productivity
If cells are cultured under hypoxic andhyperbaric conditions (1-15% oxygen, up to 2 PSI pressure), then cytokine expression is altered and cytotoxicity is enhanced, but the incubator requires independent regulation of multiple atmospheric parameters
Solution Approach 1:
The patent implements parameter changes by establishing specific ranges for oxygen (1-15%) and pressure (0-2 PSI above atmospheric) to optimize immune cell cytotoxicity. These controlled parameter changes enhance the productivity and therapeutic potential of the cultured cells while maintaining manageable incubator requirements.
Solution Approach 2:
The patent applies dynamics by allowing flexible adjustment of oxygen and pressure parameters within defined ranges rather than fixing them at single values. This dynamic approach enables optimization of cytokine expression and cell cytotoxicity while providing operational flexibility for the incubator system.
Data Source
AI summary
The present disclosure relates to a method of modulating a phenotype a source population of cells to assume a desired phenotype. The method includes culturing the source cell population within an incubator configured to regulate the variable atmospheric parameters of oxygen level and total atmospheric pressure level.