Cell Culture Bag Segmentation for Uniform Gas Distribution
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Solution Overview
Problem
Current cell culture bags face challenges in maintaining a homogeneous culture environment as the scale of cell culture increases, leading to inadequate gas supply and strength issues, which can result in inhomogeneous cell quality due to insufficient circulation and division of storage space.
Innovation Solution
A cell culture bag design featuring multiple tubular portions with gas permeability, separated by partition walls and communication portions that allow gas exchange, ensuring uniform gas distribution and circulation, while maintaining structural integrity through specific diameter and length configurations and port placement.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Volume of stationary object
If the volume of the cell culture bag is increased to increase the scale of cell culture, then the capacity to culture cells is improved, but the bottom portion of the bag expands and gas supply to the center portion becomes insufficient
Solution Approach 1:
The cell culture bag is divided into multiple tubular portions (first, second, third tubular portions) separated by partition walls. This segmentation allows each portion to have controlled expansion and ensures uniform gas distribution throughout the entire volume, preventing the bottom portion from expanding excessively while maintaining adequate gas supply to the center portion.
2Quantity of substance
If a large amount of culture solution is stored in the cell culture bag to increase culture scale, then the capacity to culture cells is improved, but the strength of the cell culture bag becomes insufficient
Solution Approach 1:
The bag is segmented into multiple tubular portions with partition walls, which distributes the weight and pressure of the culture solution across multiple structural sections. This segmentation enhances the overall strength and prevents localized weakening of the bag wall.
Solution Approach 2:
The cell culture bag is constructed from a flexible plastic film with controlled thickness and strength properties. The flexible material allows the bag to accommodate large volumes of culture solution while maintaining sufficient strength through its elastic properties and structured design.
3Reliability
If the storage space of the cell culture bag is divided into a plurality of regions to solve gas supply and strength issues, then the gas distribution is improved, but circulation of cell suspension or culture solution between regions may be inhibited
Solution Approach 1:
The tubular portions are connected through communication portions that allow the passage of cell suspension and culture solution between adjacent tubular portions. This merging of functions enables both regions to be separated for structural integrity and gas distribution, while also being connected for effective circulation and homogeneity maintenance.
4Strength
If heat sealing is used to form multiple cylinders in the cell culture bag to divide storage space, then the structural integrity is improved, but the environment of culturing cells easily becomes inhomogeneous
Solution Approach 1:
The bag is divided into tubular portions with partition walls that maintain structural integrity while allowing controlled circulation. The segmentation is designed to prevent excessive expansion in any single region while maintaining homogeneity through proper communication between portions.
Solution Approach 2:
The partition walls and communication portions are strategically positioned to create local variations in structure and function. This local quality differentiation allows each tubular portion to have optimized characteristics for both structural strength and culture environment homogeneity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This design effectively suppresses the deterioration of cell homogeneity at larger scales, ensuring uniform cell quality by promoting gas exchange and circulation, and maintaining the structural integrity of the culture bag.
Implementation Method 1
a plurality of tubular portions having gas permeability
Implementation Method 2
communication portions which allow communication between two mutually adjacent tubular portions
Data Source
AI summary
There is provided a cell culture bag including: plural tubular portions having gas permeability in which a tube axis direction is directed to a first direction and which are arranged side by side in a second direction intersecting with the first direction and are separated from each other using a partition wall; and communication portions having gas permeability which allow communication between two mutually adjacent tubular portions of the plural tubular portions in an intermediate region R between one end and the other end of the tubular portions.