Cell Culture Bag Sensor Nesting for Aseptic Dissolved Oxygen Measurement
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Solution Overview
Problem
In cell culture processes, existing cell culture bags require separate sterilization of sensor chips attached to the bags, which can lead to inadequate sterilization and potential contamination of culture fluids, as the sensor chips are exposed and not integrated within the bag structure.
Innovation Solution
A cell culture bag design featuring a sensor chip on an external film covered by an oxygen-permeable film, allowing oxygen to pass through for measurement without direct contact with the culture fluid, eliminating the need for additional sterilization and preventing substance elution into the fluid.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If sensor chip is attached to the cell culture bag with adhesive layer, then the sensor can measure dissolved oxygen concentration, but the sensor chip is exposed and requires separate sterilization which may lead to inadequate sterilization and potential contamination
Solution Approach 1:
The sensor chip is embedded within a pocket structure formed by the first film layer, creating a nested configuration where the sensor chip is housed inside the bag structure rather than being externally attached. This nesting approach integrates the sensor into the sterile environment of the bag, eliminating the need for separate sterilization of externally attached sensors while maintaining measurement capability.
Solution Approach 2:
A second film layer is introduced as an intermediary component that covers the sensor chip while allowing oxygen permeation. This intermediary layer acts as a barrier that separates the sensor chip from direct contact with the culture fluid, yet maintains the measurement function by allowing oxygen to pass through to the sensor.
2Measurement precision
If sensor chip is attached to the cell culture bag, then oxygen detection is enabled, but substances from the sensor chip may elute into the culture fluid causing contamination
Solution Approach 1:
The sensor chip is nested within a pocket structure formed by the first film layer, which provides physical containment. This nesting prevents substances from the sensor chip from eluting into the culture fluid while still allowing the sensor to detect oxygen concentrations through the film structure.
Solution Approach 2:
The second film layer serves as an intermediary barrier between the sensor chip and the culture fluid. This layer prevents direct contact and potential substance elution while maintaining oxygen permeability, thus protecting the culture fluid from contamination by sensor chip substances.
3Measurement precision
If sensor chip is exposed and attached to the bag, then measurement is possible, but additional sterilization steps are required increasing process complexity
Solution Approach 1:
The sensor chip attachment and sterilization processes are merged into a single integrated structure. The sensor chip is incorporated into the bag structure during manufacturing, allowing the entire assembly to be sterilized together as one unit, eliminating separate sterilization steps for externally attached sensors.
Solution Approach 2:
The sensor chip is nested within the pocket structure of the first film layer, creating an integrated assembly that can be sterilized as a single unit. This nesting eliminates the need for separate sterilization of externally attached components, simplifying the overall sterilization process.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Ensures aseptic conditions and prevents contamination by integrating the sensor chip within the bag structure, enhancing the reliability of dissolved oxygen concentration measurements and reducing the risk of substance elution into the culture fluid.
Implementation Method 1
a permeable film that covers a culture fluid side of the sensor chip, the permeable film permeating the object to be detected
Implementation Method 2
The oxygen-sensitive film 342 is a photo-excited substance having, for example, oxygen quenching properties
Implementation Method 3
The reflective film 343 is used to reflect the excitation light to return this excitation light to the oxygen-sensitive film 342
Implementation Method 4
The receiving optical system receives the phosphorescence emitted by the sensor chip 340 and converts the phosphorescence into an electrical signal
Data Source
AI summary
A cell culture bag is provided. The cell culture bag includes: an external film of the cell culture bag; at least one sensor chip arranged on a culture fluid side of the external film, the at least one sensor chip including a layer reacting to an object to be detected; and a permeable film that covers a culture fluid side of the sensor chip, the permeable film permeating the object to be detected.