Cell Aggregate Culture Circulation With Feedback Liquid Control
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Solution Overview
Problem
Conventional cell culture systems risk damaging or dividing cells or cell aggregates due to local pressure against filters, preventing accurate growth of cell aggregates.
Innovation Solution
A culture system that includes a culture portion with an outlet and inlet for liquid flow, a monitoring portion to track liquid components and properties, an adjustment portion to modify these, and a control portion to manage the system based on monitoring results, facilitating accurate growth of cell aggregates.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If a filter is used to separate cell culture solution from cells and cell aggregates, then the cell culture solution can be effectively filtered, but the cells or cell aggregates may be locally pressed against the filter and damaged or divided
Solution Approach 1:
The patent introduces a circulation system as an intermediary between the culture vessel and the filter. The circulation system includes a circulation pump, circulation tube, and bubble separator that work together to gently transport and separate cell aggregates from the culture solution without direct contact with the filter, thereby preventing cell damage while maintaining effective separation.
Solution Approach 2:
The patent employs hydraulic principles by using a circulation pump to create controlled fluid flow through the circulation tube. The liquid culture is circulated through the system under controlled pressure, allowing gentle separation of cell aggregates from the culture solution without mechanical contact that would cause damage.
2Productivity
If cells are cultured in a liquid medium, then cell growth can be maintained, but cell aggregates may be damaged or divided due to local pressure
Solution Approach 1:
The patent implements a dynamic circulation system where the liquid culture is continuously moved through the circulation tube at controlled speeds. The circulation pump adjusts the flow rate dynamically to maintain optimal conditions for cell aggregate growth while preventing damage from excessive pressure or agitation.
Solution Approach 2:
The patent incorporates a control unit that monitors the cell culture conditions and adjusts the circulation pump operation accordingly. The control unit receives feedback about the culture state and modifies the circulation parameters to maintain cell aggregate integrity while maximizing growth productivity.
Data Source
AI summary
Provided is a culture system capable of generating a sufficiently grown cell aggregate. A culture system that forms a cell aggregate by liquid culture includes: a culture portion that contains a liquid and has an outlet through which the liquid is discharged and an inlet through which the liquid is introduced; a path portion that allows the liquid to flow between the outlet and the inlet; a monitoring portion capable of monitoring a component in the liquid and/or properties of the liquid in at least one of the culture portion and the path portion; an adjustment portion capable of adjusting the component in the liquid and/or the properties of the liquid in at least one of the culture portion and the path portion; and a control portion capable of controlling the adjustment portion on the basis of a monitoring result of the monitoring portion.