Cell Culture Medium Control Using Lactic Acid Feedback
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Solution Overview
Problem
Existing cell culturing systems face issues with inconsistent cell expansion and unstable product quality due to inadequate control of culture medium supply, leading to sporadic cell counts and increased costs.
Innovation Solution
A biological component treatment system and device that includes a measurement unit for lactic acid detection and a control unit to calculate cell numbers based on lactic acid levels, adjusting culture medium supply to stabilize product quality and reduce costs.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Manufacturing precision
If the supplied amount of culture medium is gradually increased in a stepwise manner according to the passage of time, then the cell culture process can be maintained with simple control, but the number of cells obtained becomes sporadic and product quality becomes unstable
Solution Approach 1:
The system measures lactic acid concentration in the culture medium and uses this information as feedback to dynamically adjust the culture medium supply amount. The control unit calculates the supply amount based on the relationship between lactic acid concentration and cell number, ensuring stable cell expansion and product quality while avoiding overly complex control mechanisms.
2Productivity
If the supplied amount of culture medium is increased to support cell expansion, then cell growth can be maintained, but wasteful usage of culture medium occurs
Solution Approach 1:
The system uses lactic acid concentration measurements as feedback to precisely control culture medium supply. Since lactic acid concentration correlates with cell number and metabolic activity, the control unit can accurately determine the minimum necessary culture medium amount to support current cell growth, eliminating wasteful excess supply while maintaining productivity.
Solution Approach 2:
The system changes the control parameter from time-based stepwise increase to lactic acid concentration-based dynamic adjustment. This parameter change enables precise matching of culture medium supply to actual cell needs, improving both productivity and reducing substance loss.
3Manufacturing precision
If lactic acid concentration is used to calculate cell number and control culture medium supply, then product quality can be stabilized and manufacturing costs reduced, but measurement and control systems must be implemented
Solution Approach 1:
The system implements a feedback control mechanism where lactic acid concentration is measured and used to calculate cell number, which then determines culture medium supply. This feedback loop stabilizes cell count and product quality while keeping the control logic relatively simple by using a single measurable parameter (lactic acid concentration) that correlates with cell metabolism.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Stabilizes cell expansion outcomes and reduces manufacturing costs by dynamically controlling culture medium supply based on lactic acid measurements, ensuring consistent cell counts and improved product quality.
Implementation Method 1
a measurement unit configured to perform a measurement of information in relation to lactic acid contained in the culture medium
Data Source
AI summary
A biological component treatment system is equipped with a biological component kit having a path through which a culture medium is capable of flowing, and a biological component treatment device that controls a flowing state of the culture medium. A method for culturing cells includes performing a measurement of information in relation to lactic acid, calculating a number of cells during culturing of cells on the basis of the obtained information in relation to lactic acid and calibration information, and controlling supply of the culture medium to the cells on the basis of the number of cells.