Cell Culture Apparatus Maturity Sorting for Tissue Quality
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Solution Overview
Problem
The challenge is to stably supply living tissues for transplantation with consistent quality, as the maturity of cells during cultivation varies, leading to deteriorated tissue quality when formed into sheets or three-dimensional structures.
Innovation Solution
A cell culture device and method that includes multiple cell culture portions with a maturity evaluation system to assess cell growth, a movement mechanism to transfer cells between portions based on evaluation criteria, and adjustable culture conditions to ensure even maturity across all units, thereby stabilizing tissue quality.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Device complexity
If cells are cultured in a single cell culture portion, then the culture process is simple, but the maturity of cells varies during cultivation leading to deteriorated tissue quality
Solution Approach 1:
The cell culture system is divided into multiple cell culture portions (first, second, third portions) with different culture conditions. Cells are segmented and cultured in different portions based on their maturity stage, allowing simultaneous cultivation under optimized conditions for each stage while maintaining overall system simplicity.
Solution Approach 2:
Different culture conditions are applied to different cell culture portions to match the specific needs of cells at various maturity stages. The first portion uses conditions optimized for early-stage cells, the second for intermediate-stage cells, and the third for late-stage cells, ensuring each group receives locally appropriate cultivation environment.
2Ease of manufacture
If cells with varying maturity are made into sheets or three-dimensional structures, then the cultivation process is straightforward, but the qualities of living tissues are deteriorated
Solution Approach 1:
Cells are pre-sorted by maturity stage through automatic determination and movement to appropriate culture portions before tissue formation. This preliminary sorting ensures that only cells with appropriate and consistent maturity are grouped together for sheet or three-dimensional structure formation, guaranteeing reliable tissue quality while maintaining ease of manufacture.
Solution Approach 2:
The system uses automatic determination of target cells based on maturity evaluation to control cell movement between portions. This feedback mechanism continuously monitors cell maturity and adjusts cell distribution accordingly, ensuring that cells used for tissue formation have consistent quality while keeping the manufacturing process straightforward.
3Manufacturing precision
If automatic determination and movement of cells based on maturity is implemented, then tissue quality consistency is improved, but the device complexity increases
Solution Approach 1:
The system enables self-service through automatic determination of target cells and automated movement between culture portions based on maturity evaluation. The device autonomously sorts and redistributes cells without manual intervention, achieving uniform cell maturity while the added complexity is offset by reduced operational burden and improved consistency.
Data Source
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AI summary
A cell culture device and a cell culture method that enable living tissues for transplantation having constant qualities to be stably supplied as products are provided. A plurality of cell culture portions 10, 20, and 30 that respectively culture a plurality of cell culture units; a maturity evaluation portion 50 that respectively evaluates maturity of the respective cell culture units in the respective cell culture portions 10, 20, and 30; and a movement target cell determining portion 60 that determines a cell culture unit that does not satisfy a predetermined condition as a target to be moved to another cell culture portion in a case where the cell culture unit of which maturity does not satisfy the predetermined condition among a plurality of cell culture units cultured in any one cell culture portion of the plurality of cell culture portions 10, 20, and 30 exists are provided.