Cell Culture Medium for Lymphocyte Expansion
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Solution Overview
Problem
Current cell culture media for immune cell expansion in immunotherapy do not consistently achieve sufficient proliferation rates and quality, requiring multiple serum batch testing to ensure quality standards.
Innovation Solution
A cell culture medium with predefined quality factors such as estradiol, cortisol, IGF-1, insulin, and specific cytokine concentrations, along with NY-ESO-1 peptide, IL-2, IL-15, and IL-21, is formulated and prepared by selecting blood products based on measured concentrations to ensure optimal lymphocyte expansion.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If conventional cell culture media with serum are used for lymphocyte expansion, then the media provide a natural environment similar to the body, but the proliferation rate and activity of lymphocytes are insufficient and results are unpredictable
Solution Approach 1:
The patent applies parameter changes by precisely controlling the concentrations of multiple quality factors including estradiol (86 pmol/l), cortisol (231 nmol/l), IGF-1 (157 μg/l), insulin (7.2 mU/l), and various cytokines. This systematic adjustment of biochemical parameters transforms conventional serum-based media into a optimized formulation that achieves both high proliferation rates and predictable results for lymphocyte expansion
Solution Approach 2:
The invention creates a composite culture medium that combines multiple blood-derived components (serum and plasma) with specifically supplemented growth factors and cytokines. This composite formulation integrates the natural environment benefits of serum with the controlled efficacy of defined growth factors, resolving the contradiction between natural compatibility and reliable performance
2Reliability
If multiple batches of serum are tested to identify quality, then quality standards can be met, but the process requires extensive testing time and resources
Solution Approach 1:
The patent implements preliminary action by pre-defining specific concentration ranges for quality factors that have been established to ensure optimal lymphocyte expansion. Rather than testing multiple serum batches to discover suitable quality parameters, the medium is formulated in advance with optimized concentrations of estradiol, cortisol, IGF-1, insulin, and cytokines, eliminating the need for extensive batch testing while maintaining high quality standards
Data Source
AI summary
The present invention provides a method for preparing a cell culture medium comprising a mixture of blood products from two or more donors, comprising the steps of: a) providing at least a first blood product from a first donor; b) measuring the concentration of at least one quality factor in the first blood product; c) comparing the measured concentration of a quality factor to a concentration range predefined for the quality factor; d) selecting the first blood product for the cell culture medium if the concentration measured for the quality factor is in the predefined range and optionally converting the first selected blood product into a first processed blood product or else unselecting the first blood product.