Pre-Coated Cell Culture Surface for Easier Adherent Cell Seeding
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Solution Overview
Problem
Conventional cell culture devices for adherent cells are cumbersome due to the need for cumbersome seeding, surface modification, and washing operations, which hinder mass productivity.
Innovation Solution
A cell culture device with a body and a cover member forming culture spaces, where the cover member is surface-modified to facilitate smooth cell attachment, and includes a nanofiber membrane or plasma-treated film to support cell culture, with features like cutout portions and welding protrusions for enhanced sealing and identification.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of operation
If conventional cell culture devices are used for adherent cells, then cells can be cultured on a support surface, but the seeding operation becomes very cumbersome due to required surface modification and washing steps
Solution Approach 1:
The culture surface is pre-coated with coating solution during the manufacturing process before the device is used for cell culture. This preliminary action eliminates the need for surface modification and washing operations when actually using the device for seeding, thereby simplifying the seeding operation while maintaining the ability to culture adherent cells effectively
2Ease of operation
If surface modification and washing operations are performed for each culture, then cell attachment is facilitated, but mass productivity is reduced due to the time-consuming process
Solution Approach 1:
The coating solution is applied to the culture surface during manufacturing rather than during each culture operation. This allows multiple culture devices to be prepared in advance with pre-coated surfaces, enabling mass production and high-throughput applications without repeating the time-consuming surface modification and washing steps for each culture
3Ease of operation
If coating solution is injected and dried for each culture, then smooth cell attachment is achieved, but the operation becomes very time-consuming
Solution Approach 1:
The coating solution is applied and dried during the manufacturing process to create a permanently coated culture surface. This preliminary coating action is performed once during production rather than repeatedly during each culture operation, thereby eliminating the time loss associated with surface modification and drying steps while ensuring smooth cell attachment is maintained
Solution Approach 2:
The invention employs a disposable culture device with pre-coated surface that can be used once and then discarded. This approach eliminates the need for repeated surface modification and washing operations, saving time and resources. The low cost of the disposable device makes this economically viable compared to reusing and re-coating expensive equipment
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Facilitates easy seeding operations, reduces production costs, and maintains mass productivity by simplifying the seeding process while ensuring smooth cell attachment and efficient nutrient supply.
Implementation Method 1
the cover member may include a motif-coated plate-like nanofiber membrane, and a supporting member attached to one surface of the nanofiber membrane through an adhesive layer to support the nanofiber membrane, and an exposed surface of the nanofiber membrane may form the culture surface
Implementation Method 2
the cover member may be a plasma-treated plate-like film member, and one surface of the film member may form the culture surface
Implementation Method 3
a supporting member attached to one surface of the nanofiber membrane through an adhesive layer to support the nanofiber membrane
Data Source
AI summary
A cell culture device is provided. The cell culture device according to an exemplary embodiment of the present invention comprises: a body which includes a plurality of culture spaces formed therethrough in the height direction; and a plate-shaped cover member which is fixed to the bottom surface of the body while covering the open bottom portions of the culture spaces to thus form a culture surface where cells are cultured, and is surface-modified so as to allow the cells to be effectively attached to the culture surface.